新型植物根际细菌弗氏柠檬酸杆菌对食品真菌毒素脱氧雪腐镰刀菌烯醇的降解和生防作用的体外评价。
In-vitro assessment of a novel plant rhizobacterium, Citrobacter freundii, for degrading and biocontrol of food mycotoxin deoxynivalenol.
机构信息
School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.
School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China.
出版信息
Toxicon. 2023 May;227:107095. doi: 10.1016/j.toxicon.2023.107095. Epub 2023 Mar 25.
Deoxynivalenol (DON) is one of the most harmful and well-known toxins present in food and animal feed throughout the world. Citrobacter freundii (C. freundii-ON077584), a novel DON-degrading strain, was isolated from rice root-linked soil samples. The degrading properties, including DON concentrations, incubation pH, incubation temperatures, bacterial concentrations, and acid treatment effect on degradation, were evaluated. At pH 7 and an incubation temperature of 37 °C, C. freundii demonstrated the capability to degrade more than 90% of DON. The degraded products of DON were identified as 3-keto-DON and DOM-1, which were confirmed by High Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography hyphenated with Tandem Mass Spectrometry (UPLC-MS/MS) analyses. The mechanism of DON degradation into 3-keto-DON and DOM-1 by this bacterial strain will be further explored to identify and purify novel degrading enzymes that can be cloned to the microorganism and added to the animal feed to degrade the DON in the digestion tract.
脱氧雪腐镰刀菌烯醇（DON）是一种在全世界的食品和动物饲料中存在的最具危害性和广为人知的毒素之一。从水稻根际土壤样本中分离到一种新型的 DON 降解菌株，柠檬酸杆菌（C. freundii-ON077584）。评估了其降解特性，包括 DON 浓度、孵育 pH 值、孵育温度、细菌浓度和酸处理对降解的影响。在 pH 值为 7 和孵育温度为 37°C 的条件下，C. freundii 能够降解超过 90%的 DON。DON 的降解产物被鉴定为 3-酮-DON 和 DOM-1，这通过高效液相色谱（HPLC）和与串联质谱（UPLC-MS/MS）联用的超高效液相色谱法分析得到了证实。将进一步探索该细菌菌株将 DON 降解为 3-酮-DON 和 DOM-1 的机制，以鉴定和纯化新型降解酶，并将其克隆到微生物中，添加到动物饲料中以降解消化道中的 DON。
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