School of Public Health, Baotou Medical College, Baotou, 014040, China; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China.
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China.
Virol Sin. 2023 Apr;38(2):327-333. doi: 10.1016/j.virs.2023.03.006. Epub 2023 Mar 25.
• Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity. • The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained. • Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated. • Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.
• 对猴痘病毒进行全基因组测序对于监测新出现的变异株和评估其潜在致病性至关重要。
• 简要解释宏基因组二代测序的关键步骤，包括核酸提取、文库制备、测序和数据分析。
• 讨论了样品预处理、病毒富集和测序平台选择的优化策略。
• 强烈建议同时进行下一代测序和第三代测序。
