Li Liping, Cao Yongxin, Ippolito James A, Xing Weiqin, Qiu Kunyan, Li Hongbo, Zhao Di, Wang Yali, Wang Yale
School of the Environment, Henan University of Technology, Zhengzhou, Henan 450001, China; Henan International Joint Laboratory of Environmental Pollution, Remediation and Grain Quality Security, Zhengzhou, Henan 450001, China.
School of the Environment, Henan University of Technology, Zhengzhou, Henan 450001, China; Henan International Joint Laboratory of Environmental Pollution, Remediation and Grain Quality Security, Zhengzhou, Henan 450001, China.
Sci Total Environ. 2023 Jun 25;879:163036. doi: 10.1016/j.scitotenv.2023.163036. Epub 2023 Mar 25.
Geophagy is common for free-range chickens, however, the relative bioavailability (RBA) of heavy metals in contaminated soils consumed by chickens has not fully investigated. In this work, chickens were fed diets increasingly spiked with a contaminated soil (Cd = 105, Pb = 4840 mg kg; 3, 5, 10, 20 and 30 % of overall feed by weight), or Cd/Pb reagent spikes (from CdCl or Pb(Ac)), for 23 d. After the study period, chicken liver, kidney, femur and gizzard samples were analyzed for Cd and Pb concentrations, and organ/tissue metal concentrations were used to calculate Cd and Pb RBA. Linear dose response curves (DRCs) were established for both Cd/Pb reagents-spiked and soil-spiked treatments. Femur Cd concentrations of soil-spiked treatments were two times of Cd-spiked treatments with similar feed Cd levels, while feed spiked with Cd or Pb also resulted in elevated Pb or Cd concentrations in some organ/tissues. Metal RBA was calculated using three different methods. Most Cd and Pb RBA values were in the range 50-70 %, with the chicken gizzard as a potential endpoint for bioaccessible Cd and Pb. Cadmium and Pb bioavailability values can help with more precise estimation of Cd and Pb accumulation in chicken following heavy metal-contaminated soil ingestion, with overall results helping to protect human health.
食土癖在散养鸡中很常见，然而，鸡摄入的受污染土壤中重金属的相对生物利用度（RBA）尚未得到充分研究。在这项研究中，给鸡喂食添加了受污染土壤（镉含量为105、铅含量为4840毫克/千克；占总饲料重量的3%、5%、10%、20%和30%）或镉/铅试剂添加物（来自氯化镉或醋酸铅）的日粮，持续23天。研究期结束后，分析鸡的肝脏、肾脏、股骨和砂囊样本中的镉和铅浓度，并使用器官/组织金属浓度来计算镉和铅的RBA。为添加镉/铅试剂和添加土壤的处理建立了线性剂量反应曲线（DRC）。在饲料镉水平相似的情况下，添加土壤处理的股骨镉浓度是添加镉处理的两倍，而添加镉或铅的饲料也导致一些器官/组织中的铅或镉浓度升高。使用三种不同方法计算金属RBA。大多数镉和铅的RBA值在50%-70%的范围内，鸡砂囊是生物可利用镉和铅的潜在终点。镉和铅的生物利用度值有助于更精确地估计鸡摄入重金属污染土壤后镉和铅的积累情况，总体结果有助于保护人类健康。
