Department of Spine, Wangjing Hospital, China Academy of Chinese Medical Sciences, Huajiadi Street, Chaoyang District, Beijing, 100102, China.
School of Traditional Chinese Medicine & School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Sci Rep. 2023 Mar 27;13(1):4991. doi: 10.1038/s41598-023-31541-8.
Chronic gastritis (CG) and osteoporosis (OP) are common and occult diseases in the elderly and the relationship of these two diseases have been increasingly exposed. We aimed to explore the clinical characteristics and shared mechanisms of CG patients combined with OP. In the cross-sectional study, all participants were selected from BEYOND study. The CG patients were included and classified into two groups, namely OP group and non-OP group. Univariable and multivariable logistic regression methods were used to evaluate the influencing factors. Furthermore, CG and OP-related genes were obtained from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified using the GEO2R tool and the Venny platform. Protein-protein interaction information was obtained by inputting the intersection targets into the STRING database. The PPI network was constructed by Cytoscape v3.6.0 software again, and the key genes were screened out according to the degree value. Gene function enrichment of DEGs was performed by Webgestalt online tool. One hundred and thirty CG patients were finally included in this study. Univariate correlation analysis showed that age, gender, BMI and coffee were the potential influencing factors for the comorbidity (P < 0.05). Multivariate Logistic regression model found that smoking history, serum PTH and serum β-CTX were positively correlated with OP in CG patients, while serum P1NP and eating fruit had an negative relationship with OP in CG patients. In studies of the shared mechanisms, a total of 76 intersection genes were identified between CG and OP, including CD163, CD14, CCR1, CYBB, CXCL10, SIGLEC1, LILRB2, IGSF6, MS4A6A and CCL8 as the core genes. The biological processes closely related to the occurrence and development of CG and OP mainly involved Ferroptosis, Toll-like receptor signaling pathway, Legionellosis and Chemokine signaling pathway. Our study firstly identified the possible associated factors with OP in the patients with CG, and mined the core genes and related pathways that could be used as biomarkers or potential therapeutic targets to reveal the shared mechanisms.
慢性胃炎（CG）和骨质疏松症（OP）是老年人中常见的隐匿性疾病，这两种疾病之间的关系日益受到关注。本研究旨在探讨合并 OP 的 CG 患者的临床特征和共同发病机制。本横断面研究从 BEYOND 研究中选取所有参与者。纳入 CG 患者，并分为 OP 组和非 OP 组。采用单变量和多变量逻辑回归方法评估影响因素。此外，从基因表达综合数据库（GEO）中获取 CG 和 OP 相关基因。使用 GEO2R 工具和 Venny 平台鉴定差异表达基因（DEGs）。将交集靶基因输入 STRING 数据库获取蛋白质-蛋白质相互作用信息。再次使用 Cytoscape v3.6.0 软件构建 PPI 网络，并根据度数值筛选关键基因。通过 Webgestalt 在线工具对 DEGs 进行基因功能富集分析。最终纳入 130 例 CG 患者。单因素相关性分析表明，年龄、性别、BMI 和咖啡是合并症的潜在影响因素（P<0.05）。多变量 Logistic 回归模型发现，CG 患者中吸烟史、血清 PTH 和血清 β-CTX 与 OP 呈正相关，而血清 P1NP 和吃水果与 CG 患者的 OP 呈负相关。在共同发病机制研究中，共鉴定出 CG 和 OP 之间的 76 个交集基因，包括 CD163、CD14、CCR1、CYBB、CXCL10、SIGLEC1、LILRB2、IGSF6、MS4A6A 和 CCL8 等核心基因。与 CG 和 OP 发生发展密切相关的生物学过程主要涉及铁死亡、Toll 样受体信号通路、军团菌病和趋化因子信号通路。本研究首次鉴定了 CG 患者合并 OP 的可能相关因素，并挖掘了可能作为生物标志物或潜在治疗靶点的核心基因和相关通路，以揭示共同发病机制。
