Chen Haiyan, Sun Qi, Zhang Cangang, She Junjun, Cao Shuai, Cao Meng, Zhang Nana, Adiila Ayarick Vivian, Zhong Jinjin, Yao Chengyun, Wang Yili, Xia Hongping, Lan Linhua
Institute for Cancer Research, School of Basic Medical Science of Xi'an Jiaotong University, Xi'an, China.
Department of Pathology, School of Basic Medical Sciences and Sir Run Run Hospital and Key Laboratory of Antibody Technique of National Health Commission, Nanjing Medical University, Nanjing, China.
Front Mol Biosci. 2021 Dec 7;8:756085. doi: 10.3389/fmolb.2021.756085. eCollection 2021.
Gastric cancer (GC) is rampant around the world. Most of the GC cases are detected in advanced stages with poor prognosis. The identification of marker genes for early diagnosis is of great significance. Studying the tumor environment is helpful to acknowledge the process of tumorigenesis, development, and metastasis. Twenty-two kinds of immune cells were calculated by CIBERSORT from Gene Expression Omnibus (GEO) database. Subsequently, higher infiltration of macrophages M0 was discovered in GC compared with normal tissues. WGCNA was utilized to construct the network and then identify key modules and genes related to macrophages in TCGA. Finally, 18 hub genes were verified. In the PPI bar chart, the top 3 genes were chosen as hub genes involved in most pathways. On the TIMER and THPA websites, it is verified that the expression levels of CYBB, CD86, and C3AR1 genes in tumor tissues were higher than those in normal tissues. These genes may work as biomarkers or targets for accurate diagnosis and treatment of GC in the future. Our findings may be a new strategy for the treatment of GC.
胃癌（GC）在全球范围内肆虐。大多数GC病例在晚期被检测出，预后较差。鉴定用于早期诊断的标志物基因具有重要意义。研究肿瘤微环境有助于了解肿瘤发生、发展和转移的过程。通过CIBERSORT从基因表达综合数据库（GEO）中计算出22种免疫细胞。随后，发现与正常组织相比，GC中M0巨噬细胞的浸润更高。利用加权基因共表达网络分析（WGCNA）构建网络，然后在癌症基因组图谱（TCGA）中鉴定与巨噬细胞相关的关键模块和基因。最后，验证了18个枢纽基因。在蛋白质-蛋白质相互作用（PPI）柱状图中，选择排名前3的基因作为参与大多数通路的枢纽基因。在肿瘤免疫评估资源（TIMER）和人类蛋白质图谱（THPA）网站上，验证了肿瘤组织中CYBB、CD86和C3AR1基因的表达水平高于正常组织。这些基因可能在未来作为GC精确诊断和治疗的生物标志物或靶点。我们的研究结果可能是GC治疗的一种新策略。
