糖尿病状态持续时间改变了链脲佐菌素诱导的 1 型糖尿病大鼠模型中血小板指数与基线值的差异。

Duration of the diabetic state altered platelet indices from baseline values in a streptozotocin-induced rat model for type 1 diabetes mellitus.

机构信息

Faculty of Veterinary Medicine, Department of Pathobiology, Semnan University, Semnan, Iran.

Faculty of Veterinary Medicine, Department of Clinical Sciences, Semnan University, Semnan, Iran.

出版信息

Vet Clin Pathol. 2023 Jun;52(2):236-242. doi: 10.1111/vcp.13229. Epub 2023 Mar 27.

DOI:10.1111/vcp.13229

PMID:36973508

Abstract

BACKGROUND

Changes in platelet indices in naturally occurring type I diabetes mellitus (T1DM) have been described in several studies. In this study, platelet indices such as platelet count (PLT), plateletcrit (PCT), mean platelet volume (MPV), platelet distribution width (PDW), and MPV to PLT ratio were investigated according to diabetic duration after streptozotocin (STZ)-induced T1DM, as well as for their correlation with glucose.

METHODS

Forty healthy adult Wistar rats were randomly assigned to four experimental groups of ten (5 rats of each sex), including the control group, the 7, 14, and 28 days diabetic groups (D7, D14, and D28, respectively).

RESULTS

In diabetic groups, plasma glucose was significantly higher than in control (P < 0.01). D7, D14, and D28 groups presented significantly lower PLT than the control (P < 0. 01). A significant decrease in PCT was observed in D14 and D28 females (P < 0.05). Mean platelet volume was significantly higher in the D28 group than in to control. D28 females also showed a significant difference in PLT, MPV, and the MPV-to-PLT ratio compared with D7 females (P < 0.05). A comparison between D28 females and males showed a significant difference in PDW (P < 0.05). Both females and males showed a significant correlation between glucose and PLT, PCT, MPV, and the MPV-to-PLT ratio.

CONCLUSIONS

Platelet indices change significantly with the duration of diabetes compared with the baseline values, and female and male rats did not have significant differences in platelet indices in any period except the 28 days.

摘要

背景

已有多项研究描述了自然发生的 1 型糖尿病（T1DM）中血小板指数的变化。在这项研究中，根据链脲佐菌素（STZ）诱导的 T1DM 后的糖尿病持续时间，研究了血小板计数（PLT）、血小板压积（PCT）、平均血小板体积（MPV）、血小板分布宽度（PDW）和 MPV 与 PLT 的比值等血小板指数，并研究了它们与血糖的相关性。

方法

将 40 只健康成年 Wistar 大鼠随机分为四组，每组 10 只（每组各 5 只），包括对照组、7 天组（D7）、14 天组（D14）和 28 天组（D28）。

结果

在糖尿病组中，血浆葡萄糖明显高于对照组（P<0.01）。D7、D14 和 D28 组的 PLT 明显低于对照组（P<0.01）。D14 和 D28 组雌性 PCT 显著降低（P<0.05）。D28 组的平均血小板体积明显高于对照组。与 D7 组雌性大鼠相比，D28 组雌性大鼠的 PLT、MPV 和 MPV 与 PLT 的比值也有显著差异（P<0.05）。与 D28 组雄性大鼠相比，D28 组雌性大鼠的 PDW 也有显著差异（P<0.05）。无论是雌性大鼠还是雄性大鼠，血糖与 PLT、PCT、MPV 和 MPV 与 PLT 的比值均呈显著相关。