Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, the Netherlands.
Biomedical Sciences of Cells & Systems, University of Groningen UMC Groningen, Groningen, the Netherlands.
Nat Methods. 2023 Apr;20(4):541-545. doi: 10.1038/s41592-023-01809-y. Epub 2023 Mar 27.
We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems.
我们报告了 mScarlet3 的进化，它是一种不含半胱氨酸的单体红色荧光蛋白，具有快速和完全成熟的特点，以及记录亮度、量子产率（75%）和荧光寿命（4.0ns）。mScarlet3 的晶体结构显示出一个桶状结构，其中一个头部由内部残基的大疏水区固定。mScarlet3 作为融合标签表现良好，没有明显的细胞毒性，它超过了现有的红色荧光蛋白，作为Förster 共振能量转移受体和瞬时表达系统中的报告基因。
