Kosaka H, Uozumi M
Biochim Biophys Acta. 1986 May 12;871(1):14-8. doi: 10.1016/0167-4838(86)90127-5.
Oxidation of oxyhemoglobin by nitrite is characterized by a lag period followed by an autocatalytic phase. The oxidation can be inhibited by the addition of morpholine, piperidine, triethanolamine or triethylamine (6 mM each). These amines are known to react with nitrogen dioxide to yield nitrosamine. Unexpectedly, aniline or aminopyrine (120 microM each) markedly inhibited the oxidation. These compounds, but not the other amines given above, inhibited the peroxide compound formation from methemoglobin and hydrogen peroxide. The results establish that, during the oxidation, the peroxide compound is generated and converts nitrite into nitrogen dioxide by its peroxidatic activity, resulting in an autocatalytic phase.
亚硝酸盐对氧合血红蛋白的氧化作用具有一个延迟期，随后是一个自催化阶段。加入吗啉、哌啶、三乙醇胺或三乙胺（每种6 mM）可抑制这种氧化作用。已知这些胺类会与二氧化氮反应生成亚硝胺。出乎意料的是，苯胺或氨基比林（每种120 μM）能显著抑制这种氧化作用。这些化合物而非上述其他胺类，抑制了高铁血红蛋白和过氧化氢形成过氧化物化合物。结果表明，在氧化过程中，过氧化物化合物会生成，并通过其过氧化物酶活性将亚硝酸盐转化为二氧化氮，从而导致自催化阶段。
