Ghoushouni Sevda, Sharifi Akbar, Zafari Venus, Zarredar Habib, Seyedrezazadeh Ensiyeh
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Basic Oncology, Health Institute of Ege University, Izmir, Turkey.
J Res Med Sci. 2023 Feb 21;28:11. doi: 10.4103/jrms.jrms_860_21. eCollection 2023.
Understanding the contributing of influence inflammatory biomarkers in asthmatic patients with metabolic syndrome is more important. Whereby, the present study considering the important association of NADPH oxidase4 (NOX4) and Toll- like receptor4 (TLR4) in the respiratory inflammatory responses in asthmatic patients with metabolic syndrome (AS-MetS) and asthmatic (AS) patients.
In this case-control study, 30 AS and 34 AS-MetS patients were enrolled. The Peripheral blood mononuclear cells (PBMCs) mRNA and protein levels of TLR4 and NOX4 were measured by qRT-PCR and western blot, respectively. Then their correlation was evaluated.
The significant down-regulation of mRNA and protein PBMCs expression levels of TLR4 were observed in the AS-MetS group in comparison to AS one (=0.03), but the NOX4 expression was non-significant. Additionally, the significant correlation was exhibited between mRNA expression levels of NOX4 and TLR4 in both AS-MetS (= 0.440, =0.009) and AS groups (=0.909, =0.0001). The association between TLR4 mRNA level and triglyceride in AS-MetS group (=0.454, =0.008,) and also white blood cells (WBC) in AS group (= -0.507, =0.006,) were significant.
The metabolic syndrome can significantly influence the expressions of TLR4 in AS-MetS. This study indicated that TLR4 and NOX4 altogether may provide valuable molecular knowledge of their relation with metabolic syndrome criteria for finding major pathways in different phenotype of asthma.
了解炎症生物标志物在患有代谢综合征的哮喘患者中的作用更为重要。因此，本研究考虑了NADPH氧化酶4（NOX4）和Toll样受体4（TLR4）在患有代谢综合征的哮喘患者（AS-MetS）和哮喘患者（AS）的呼吸道炎症反应中的重要关联。
在这项病例对照研究中，纳入了30例AS患者和34例AS-MetS患者。分别通过qRT-PCR和蛋白质印迹法测量外周血单核细胞（PBMCs）中TLR4和NOX4的mRNA和蛋白质水平。然后评估它们之间的相关性。
与AS组相比，AS-MetS组中TLR4的mRNA和蛋白质PBMCs表达水平显著下调（P=0.03），但NOX4表达无显著差异。此外，在AS-MetS组（r=0.440，P=0.009）和AS组（r=0.909，P=0.0001）中，NOX4和TLR4的mRNA表达水平之间均呈现显著相关性。AS-MetS组中TLR4 mRNA水平与甘油三酯之间（r=0.454，P=0.008）以及AS组中TLR4 mRNA水平与白细胞（WBC）之间（r=-0.507，P=0.006）的关联均具有显著性。
代谢综合征可显著影响AS-MetS中TLR4的表达。本研究表明，TLR4和NOX4共同可能为其与代谢综合征标准的关系提供有价值的分子知识，以发现不同哮喘表型中的主要途径。
