Department of Pathology, Faculty of Medicine, Hasanuddin University, Indonesia..
Department of Pathology, Faculty of Medicine, Hasanuddin University, Indonesia.
Asian Pac J Cancer Prev. 2023 Mar 1;24(3):929-934. doi: 10.31557/APJCP.2023.24.3.929.
The aim of this study was to analyze the expression of EpCAM in the colorectal adenocarcinoma.
This study used a cross-sectional design. One hundred and thirteen paraffin embedded block of Colorectal Adenocarcinoma were assessed using anti-EpCAM/Epithelial Specific Antigen (Ber-EP4) mouse monoclonal antibody and their expression were performed using Olympus CX-43 light microscope. The relationship between EpCAM expression with histopathological grade of colorectal adenocarcinoma, lymphovascular invasion and metastases ability were statistically analyzed by Chi-Square tests and presented in tables using SPSS 18.
From 113 samples, in samples with lymphovascular invasion there were 37 samples (32.7%) with strong expression, while those with weak expression were 19 samples (16.8%). There were 39 samples with metastases and strong expression of EpCAM (34.5%), while 21 samples with weak expression (18.6%). There was a significant relationship between the expression of cancer stem cell marker EpCAM with lymphovascular invasion and colorectal adenocarcinoma metastases (p = 0.002), but there was no significant relationship with histopathological grade (p = 0.574).
The EpCAM expression can be used as a prognostic factor, and can be considered as a predictive or an option for target therapy in colorectal adenocarcinoma.
本研究旨在分析结直肠腺癌中 EpCAM 的表达。
本研究采用横断面设计。使用抗 EpCAM/上皮特异性抗原（Ber-EP4）单克隆抗体评估 113 例结直肠腺癌石蜡包埋块，并使用 Olympus CX-43 光学显微镜进行其表达。采用卡方检验对 EpCAM 表达与结直肠腺癌组织学分级、血管淋巴管侵犯和转移能力的关系进行统计学分析，并使用 SPSS 18 以表格形式呈现。
在 113 例样本中，有 37 例（32.7%）存在血管淋巴管侵犯的样本表现为强表达，而弱表达的样本为 19 例（16.8%）。有 39 例存在转移且 EpCAM 强表达（34.5%），而弱表达的样本为 21 例（18.6%）。结直肠腺癌干细胞标志物 EpCAM 的表达与血管淋巴管侵犯和结直肠腺癌转移呈显著相关（p=0.002），但与组织学分级无显著相关性（p=0.574）。
EpCAM 的表达可作为一种预后因素，并可考虑作为结直肠腺癌预测或靶向治疗的选择。
