Department of Anatomical Pathology, Faculty of Medicine, Hasanuddin University, Indonesia.
Department of Anatomical Pathology, Faculty of Medicine, Tadulako University, Indonesia.
Asian Pac J Cancer Prev. 2024 Sep 1;25(9):3143-3149. doi: 10.31557/APJCP.2024.25.9.3143.
The aim of this study was to analyze the expression of Snail in the colorectal adenocarcinoma.
This study used a cross-sectional design. Seventy four paraffin embedded block of Colorectal Adenocarcinoma were assessed using Snail rabbit polyclonal antibody and their expression were performed using Olympus CX-43 light microscope. The relationship between Snail expression with histopathological grading, tumor budding grading, lymphovascular invasion and metastases of colorectal adenocarcinoma ability were statistically analyzed by Mann Whitney tests and presented in tables using SPSS 27.
From 74 samples examined, in samples with low grade tumor budding (n=11), there were 9 samples (81.8%) with weak expression, while those with strong expression were 2 samples (18.2%). In samples with intermediate grade tumor budding (n=28), there were 17 samples (60.7%) with weak expression, while those with strong expression were 11 samples (39.3%). In samples with high grade tumor budding (n=35), there were 13 samples (37.1%) with weak expression, while those with strong expression were 22 samples (62.9%). In samples with lymphovascular invasion (n=14), there were 10 samples (71.4%) with strong expression, while those with weak expression were 4 samples (28.6%). In samples with metastases (n=23), there were 16 samples (69.6%) with strong expression, while those with weak expression were 7 samples (30.4%). There was a significant relationship between the expression of Snail with tumor budding grade (p=0.003), lymphovascular invasion and metastases (p=<0.001), but there was no significant relationship with histopathological grade (p=0.942).
The Snail expression can be used as a prognostic factor in colorectal adenocarcinoma.
本研究旨在分析 Snail 在结直肠腺癌中的表达。
本研究采用横断面设计。使用 Snail 兔多克隆抗体评估 74 例结直肠腺癌石蜡包埋块，并使用 Olympus CX-43 光学显微镜进行表达。通过 Mann Whitney 检验对 Snail 表达与结直肠腺癌组织学分级、肿瘤芽分级、淋巴血管侵犯和转移能力之间的关系进行统计学分析，并使用 SPSS 27 在表中呈现。
在 74 例检查样本中，低级别肿瘤芽（n=11）样本中，有 9 个样本（81.8%）表达较弱，而表达较强的有 2 个样本（18.2%）。在中级肿瘤芽（n=28）样本中，有 17 个样本（60.7%）表达较弱，而表达较强的有 11 个样本（39.3%）。在高级肿瘤芽（n=35）样本中，有 13 个样本（37.1%）表达较弱，而表达较强的有 22 个样本（62.9%）。在有淋巴血管侵犯（n=14）的样本中，有 10 个样本（71.4%）表达较强，而表达较弱的有 4 个样本（28.6%）。在有转移（n=23）的样本中，有 16 个样本（69.6%）表达较强，而表达较弱的有 7 个样本（30.4%）。Snail 的表达与肿瘤芽分级（p=0.003）、淋巴血管侵犯和转移（p<0.001）之间存在显著关系，但与组织学分级（p=0.942）之间无显著关系。
Snail 的表达可作为结直肠腺癌的预后因素。
