School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, People's Republic of China.
Open Biol. 2023 Feb;13(2):220302. doi: 10.1098/rsob.220302. Epub 2023 Feb 22.
The intestine is a site of immune cell priming at birth. Therefore, spatial transcriptomes were performed to define how the transcriptomic landscape was spatially organized in the posterior intestine of following infection. In the healthy condition, we identified a previously unappreciated molecular regionalization of the posterior intestine. Following bacterial infection, most immune-related genes were identified in mucosa layer. Moreover, investigation of immune-related genes and genes in immune-related KEGG pathways based on spatial transcriptomes shed light on which sections of these genes are in the posterior intestine. Meanwhile, the high expression of genes related to regeneration also indicated that the posterior intestine was responding to the invasion of pathogens by constantly proliferating new cells. In addition, the increasing microbiota communities indicated that these bacteria maintained posterior intestine integrity and shaped the mucosal immune system. Taken together, spatial transcriptomes and microbiota compositions have significant implications for understanding the immune mechanism that responds to infection in the posterior intestine of , which also provides a theoretical basis for the spatial distribution of immune genes and changes in bacterial flora in other teleosts in the process of resisting pathogens.
肠道是出生时免疫细胞启动的部位。因此，进行了空间转录组学分析，以确定在 感染后，转录组图谱在后端肠道中的空间组织方式。在健康状态下，我们发现后端肠道的分子区域化以前未被认识到。在细菌感染后，大多数与免疫相关的基因在黏膜层被鉴定出来。此外，基于空间转录组学对与免疫相关的基因和免疫相关 KEGG 途径中的基因进行调查，揭示了这些基因在后端肠道中的哪些部分。同时，与再生相关的基因的高表达也表明后端肠道通过不断增殖新细胞来应对病原体的入侵。此外，微生物群落的增加表明这些细菌维持了后端肠道的完整性，并塑造了黏膜免疫系统。总之，空间转录组学和微生物群落组成对于理解后端肠道对 感染的免疫机制具有重要意义，这也为其他硬骨鱼类在抵抗病原体过程中免疫基因的空间分布和细菌菌群的变化提供了理论依据。
