Garvan-Weizmann Centre for Cellular Genomics, Garvan Institute of Medical Research, Sydney, NSW, 2010, Australia.
School of Medical Sciences, University of New South Wales, Sydney, NSW, 2006, Australia.
Mucosal Immunol. 2022 Apr;15(4):531-541. doi: 10.1038/s41385-021-00470-y. Epub 2021 Nov 30.
The intestinal immune system represents the largest collection of immune cells in the body and is continually exposed to antigens from food and the microbiota. Here we discuss the contribution of single-cell transcriptomics in shaping our understanding of this complex system. We consider the impact on resolving early intestine development, engagement with the neighbouring microbiota, diversity of intestinal immune cells, compartmentalisation within the intestines and interactions with non-immune cells. Finally, we offer a perspective on open questions about gut immunity that evolving single-cell technologies are well placed to address.
肠道免疫系统代表了体内最大的免疫细胞集合，并且不断暴露于来自食物和微生物组的抗原。在这里，我们讨论了单细胞转录组学在塑造我们对这个复杂系统的理解方面的贡献。我们考虑了其对早期肠道发育、与邻近微生物群的相互作用、肠道免疫细胞的多样性、肠道内的分隔以及与非免疫细胞的相互作用的影响。最后，我们对肠道免疫的一些悬而未决的问题提出了看法，认为不断发展的单细胞技术很有希望解决这些问题。
