Liu Zhihui, Zhu Xiaohua, Lu Qiujun, Liu Meiling, Li Haitao, Zhang Youyu, Liu Yang, Yao Shouzhuo
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Department of Chemistry, Beijing Key Laboratory for Analytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology of Ministry of Education, Tsinghua University, Beijing 100084, China.
Anal Chem. 2023 Apr 11;95(14):5911-5919. doi: 10.1021/acs.analchem.2c04997. Epub 2023 Mar 28.
The composition and activity of the gut microbiota are crucial for health management and disease treatment. Herein, we develop a rapid and robust multichannel sensor array via a recognition engineering strategy using antimicrobial agent (vancomycin, bacitracin, and lysozyme) functional gold nanoclusters and gluconamide-modified TiC MXenes, which provide superior fingerprint patterns to distinguish gut-derived bacteria. The discrimination ability of the sensor array was highly improved via the synergistic recognition between the bacteria and the various antimicrobial agents. Five gut-derived bacteria, including probiotics, neutral, and pathogenic bacteria were clearly differentiated and discriminated from the bacteria mixtures. Furthermore, the sensing system was successfully applied for the accurate classification of human colorectal cancer samples from healthy individuals rapidly (30 min) with clinically relevant specificity. The rapidity, simplicity, and economic cost of this strategy offers a robust platform for gut microbiota analysis.
肠道微生物群的组成和活性对于健康管理和疾病治疗至关重要。在此，我们通过一种识别工程策略开发了一种快速且强大的多通道传感器阵列，该策略使用抗菌剂（万古霉素、杆菌肽和溶菌酶）功能化金纳米簇和葡糖酰胺修饰的TiC MXene，它们提供了卓越的指纹图谱以区分肠道来源的细菌。通过细菌与各种抗菌剂之间的协同识别，传感器阵列的辨别能力得到了极大提高。五种肠道来源的细菌，包括益生菌、中性菌和致病菌，能够从细菌混合物中被清晰地区分和辨别出来。此外，该传感系统成功地应用于从健康个体中快速（30分钟）准确分类人类结直肠癌样本，具有临床相关的特异性。该策略的快速性、简便性和经济成本为肠道微生物群分析提供了一个强大的平台。
