School of Sensing Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
School of Life Sciences and Biotechnology, State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
Anal Chem. 2023 Apr 18;95(15):6193-6197. doi: 10.1021/acs.analchem.2c05455. Epub 2023 Mar 28.
Intrinsic fluorescence imaging (IFI) has been used for the stain-free detection of proteins in slab gel. However, complicated detection setups and small irradiation area limited the development of facile, online, and portable imaging of the whole slab gel. We here designed a quadruple UV LED array to produce even and powerful area light for direct irradiation of gel electrophoresis chip (GEC) at 275 nm. In addition, we only used a filter of 365 nm, a UV camera lens, and a CCD for IFI detection. We integrated the simple detection setup with the small GEC to construct the IFI-GEC device with a portable size of 15 × 15 × 38 cm. We detected three model proteins to demonstrate the good evenness of the LED array and the online imaging of the whole GEC. Furthermore, the reproducible IFI-GEC detection was completed within 10 min and the LOD was as low as 40 ng for lysozyme detection. All results indicated the potential of the IFI-GEC device for online and portable detection of proteins without staining.
本征荧光成像（IFI）已被用于平板凝胶中蛋白质的无染色检测。然而，复杂的检测设置和小的照射面积限制了整个平板凝胶的简便、在线和便携式成像的发展。我们在此设计了一个四重 UV LED 阵列，以产生均匀且强大的面光，用于在 275nm 下直接照射凝胶电泳芯片（GEC）。此外，我们仅使用 365nm 的滤光片、UV 相机镜头和 CCD 进行 IFI 检测。我们将简单的检测设置与小 GEC 集成在一起，构建了具有便携尺寸（15×15×38cm）的 IFI-GEC 设备。我们检测了三种模型蛋白，以证明 LED 阵列的均匀性和整个 GEC 的在线成像效果。此外，在 10 分钟内完成了可重复的 IFI-GEC 检测，溶菌酶检测的检出限低至 40ng。所有结果表明，IFI-GEC 设备具有在线和便携式无染色检测蛋白质的潜力。
