Safhi Fatmah Ahmed, Alshamrani Salha Mesfer, Alshaya Dalal Sulaiman, Hussein Mohammed A A, Abd El-Moneim Diaa
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Department of Biology, College of Science, University of Jeddah, Jeddah 21959, Saudi Arabia.
Curr Issues Mol Biol. 2023 Feb 22;45(3):1810-1819. doi: 10.3390/cimb45030116.
Banana plantation has been introduced recently to a temperate zone in the southeastern parts of Saudi Arabia (Fifa, Dhamadh, and Beesh, located in Jazan province). The introduced banana cultivars were of a clear origin without a recorded genetic background. In the current study, the genetic variability and structure of five common banana cultivars (i.e., Red, America, Indian, French, and Baladi) were analyzed using the fluorescently labeled AFLP technique. Nine different primer pairs combinations yielded 1468 loci with 88.96% polymorphism. Among all locations, high expected heterozygosity under the Hardy-Weinberg assumption was found (0.249 ± 0.003), where Dhamadh was the highest, followed by Fifa and Beesh, respectively. Based on the PCoA and Structure analysis, the samples were not clustered by location but in pairs in accordance with the cultivar's names. However, the Red banana cultivar was found to be a hybrid between the American and Indian cultivars. Based on Φ, 162 molecular markers (i.e., loci under selection) were detected among cultivars. Identifying those loci using NGS techniques can reveal the genetic bases and molecular mechanisms involved in the domestication and selection indicators among banana cultivars.
香蕉种植最近已引入沙特阿拉伯东南部的一个温带地区（位于吉赞省的菲法、达马德和比什）。引入的香蕉品种来源明确，但没有记录的遗传背景。在本研究中，使用荧光标记的AFLP技术分析了五个常见香蕉品种（即红香蕉、美国香蕉、印度香蕉、法国香蕉和巴拉迪香蕉）的遗传变异性和结构。九种不同的引物对组合产生了1468个位点，多态性为88.96%。在所有地点中，在哈迪-温伯格假设下发现了较高的预期杂合度（0.249±0.003），其中达马德最高，其次是菲法和比什。基于主坐标分析（PCoA）和结构分析，样本不是按地点聚类，而是根据品种名称成对聚类。然而，发现红香蕉品种是美国香蕉和印度香蕉品种的杂交种。基于Φ，在品种间检测到162个分子标记（即选择位点）。使用二代测序（NGS）技术鉴定这些位点可以揭示香蕉品种驯化和选择指标所涉及的遗传基础和分子机制。
