Department of Horticultural Science, Faculty of AgricSciences, University of Stellenbosch, Stellenbosch 7602, South Africa.
J Zhejiang Univ Sci B. 2010 May;11(5):332-41. doi: 10.1631/jzus.B0900310.
Banana is an important crop grown in Oman and there is a dearth of information on its genetic diversity to assist in crop breeding and improvement programs. This study employed amplified fragment length polymorphism (AFLP) to investigate the genetic variation in local banana cultivars from the southern region of Oman. Using 12 primer combinations, a total of 1094 bands were scored, of which 1012 were polymorphic. Eighty-two unique markers were identified, which revealed the distinct separation of the seven cultivars. The results obtained show that AFLP can be used to differentiate the banana cultivars. Further classification by phylogenetic, hierarchical clustering and principal component analyses showed significant differences between the clusters found with molecular markers and those clusters created by previous studies using morphological analysis. Based on the analytical results, a consensus dendrogram of the banana cultivars is presented.
香蕉是阿曼种植的重要作物,但关于其遗传多样性的信息很少,无法为作物的培育和改良计划提供帮助。本研究采用扩增片段长度多态性(AFLP)技术,对阿曼南部地区的当地香蕉品种进行了遗传变异研究。使用 12 对引物组合,共获得了 1094 个条带,其中 1012 个为多态性条带。共鉴定出 82 个独特的标记,这些标记明显区分了这 7 个品种。结果表明,AFLP 可用于区分香蕉品种。进一步的基于系统发育、层次聚类和主成分分析的分类表明,基于分子标记的聚类与基于形态学分析的聚类存在显著差异。根据分析结果,提出了一个香蕉品种的共识聚类图。