Evidence of Transmission of 210 and 247 by and , Major Malaria Vectors in Benin/West Africa.

Current diagnostic and surveillance systems in Benin are not designed to accurately identify or report non- () human malaria infections. This study aims to assess and compare the prevalence of circumsporozoite protein (CSP) antibodies of and () in s.l. in Benin. For that, mosquito collections were performed through human landing catches (HLC) and pyrethrum spray catches (PSC). The collected mosquitoes were morphologically identified, and , 210, and 247 CSP antibodies were sought in s.l. through the ELISA and polymerase chain reaction (PCR) techniques. Of the 32,773 collected mosquitoes, 20.9% were s.l., 3.9% gr., and 0.6% gr. In s.l., the sporozoite rate was 2.6% (95% CI: 2.1-3.1) for , against 0.30% (95% CI: 0.1-0.5) and 0.2% (95% CI: 0.1-0.4), respectively, for 210 and 247. sporozoite positive mosquitoes were mostly (64.35%), followed by (34.78%) and (0.86%). At the opposite, for the 210 sporozoite-positive mosquitoes, and accounted for 76.92% and 23.08%, respectively. Overall, the present study shows that is not the only species involved in malaria cases in Benin.