美国东南部对Cry1Ac和Cry2Ab2的抗性水平的幅度和范围
Magnitude and Extent of Resistance Levels to Cry1Ac and Cry2Ab2 across the Southeastern USA.
作者信息
Reisig Dominic, Buntin G David, Greene Jeremy K, Paula-Moraes Silvana V, Reay-Jones Francis, Roberts Phillip, Smith Ron, Taylor Sally V
机构信息
Department of Entomology, The Vernon James Center, North Carolina State University, Plymouth, NC 27962, USA.
Department of Entomology, University of Georgia, Tifton, GA 31793, USA.
出版信息
Insects. 2023 Mar 7;14(3):262. doi: 10.3390/insects14030262.
After resistance is first detected, continued resistance monitoring can inform decisions on how to effectively manage resistant populations. We monitored for resistance to Cry1Ac (2018 and 2019) and Cry2Ab2 (2019) from southeastern USA populations of . We collected larvae from various plant hosts, sib-mated the adults, and tested neonates using diet-overlay bioassays and compared them to susceptible populations for resistance estimates. We also compared LC values with larval survival, weight and larval inhibition at the highest dose tested using regression, and found that LC values were negatively correlated with survival for both proteins. Finally, we compared resistance rations between Cry1Ac and Cry2Ab2 during 2019. Some populations were resistant to Cry1Ac, and most were resistant to CryAb2; Cry1Ac resistance ratios were lower than Cry2Ab2 during 2019. Survival was positively correlated with larval weight inhibition for Cry2Ab. This contrasts with other studies in both the mid-southern and southeastern USA, where resistance to Cry1Ac, Cry1A.105, and Cry2Ab2 increased over time and was found in a majority of populations. This indicates that cotton expressing Cry proteins in the southeastern USA was at variable risk for damage in this region.
首次检测到抗性后，持续的抗性监测可为如何有效管理抗性种群的决策提供依据。我们监测了美国东南部种群对Cry1Ac（2018年和2019年）和Cry2Ab2（2019年）的抗性。我们从各种植物寄主上收集幼虫，让成虫进行同胞交配，并用饲料覆盖生物测定法对新生幼虫进行测试，并将它们与敏感种群进行比较以估算抗性。我们还使用回归分析比较了最高测试剂量下的LC值与幼虫存活率、体重及幼虫抑制情况，发现两种蛋白的LC值均与存活率呈负相关。最后，我们比较了2019年Cry1Ac和Cry2Ab2之间的抗性比率。一些种群对Cry1Ac有抗性，大多数种群对CryAb2有抗性；2019年Cry1Ac的抗性比率低于Cry2Ab2。Cry2Ab的存活率与幼虫体重抑制呈正相关。这与美国中南部和东南部的其他研究形成对比，在这些研究中，对Cry1Ac、Cry1A.105和Cry2Ab2的抗性随时间增加，且在大多数种群中都有发现。这表明在美国东南部表达Cry蛋白的棉花在该地区受到损害的风险各不相同。
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