Casarin Martina, Fortunato Tiago Moderno, Imran Saima Jalil, Todesco Martina, Sandrin Deborah, Marchesan Massimo, Gerosa Gino, Romanato Filippo, Bagno Andrea, Dal Moro Fabrizio, Morlacco Alessandro
Department of Surgery, Oncology and Gastroenterology, Giustiniani 2, 35128 Padua, Italy.
L.i.f.e.L.a.b. Program, Consorzio per la Ricerca Sanitaria (CORIS), Veneto Region, via N. Giustiniani 2, 35128 Padua, Italy.
J Funct Biomater. 2023 Mar 2;14(3):141. doi: 10.3390/jfb14030141.
Conduit substitutes are increasingly in demand for cardiovascular and urological applications. In cases of bladder cancer, radical cystectomy is the preferred technique: after removing the bladder, a urinary diversion has to be created using autologous bowel, but several complications are associated with intestinal resection. Thus, alternative urinary substitutes are required to avoid autologous intestinal use, preventing complications and facilitating surgical procedures. In the present paper, we are proposing the exploitation of the decellularized porcine descending aorta as a novel and original conduit substitute. After being decellularized with the use of two alternative detergents (Tergitol and Ecosurf) and sterilized, the porcine descending aorta has been investigated to assess its permeability to detergents through methylene blue dye penetration analysis and to study its composition and structure by means of histomorphometric analyses, including DNA quantification, histology, two-photon microscopy, and hydroxyproline quantification. Biomechanical tests and cytocompatibility assays with human mesenchymal stem cells have been also performed. The results obtained demonstrated that the decellularized porcine descending aorta preserves its major features to be further evaluated as a candidate material for urological applications, even though further studies have to be carried out to demonstrate its suitability for the specific application, by performing in vivo tests in the animal model.
心血管和泌尿外科应用对导管替代物的需求日益增加。在膀胱癌病例中，根治性膀胱切除术是首选技术：切除膀胱后，必须使用自体肠管进行尿流改道，但肠道切除会带来多种并发症。因此，需要替代的泌尿替代物以避免使用自体肠管，防止并发症并简化手术过程。在本文中，我们提议将去细胞猪降主动脉用作一种新颖的导管替代物。在用两种替代去污剂（Tergitol和Ecosurf）进行去细胞处理并灭菌后，对猪降主动脉进行了研究，通过亚甲蓝染料渗透分析评估其对去污剂的渗透性，并通过组织形态计量分析，包括DNA定量、组织学、双光子显微镜和羟脯氨酸定量，研究其组成和结构。还进行了生物力学测试和与人骨髓间充质干细胞的细胞相容性测定。所获得的结果表明，去细胞猪降主动脉保留了其主要特征，尽管还需要通过在动物模型中进行体内试验来进一步研究其是否适合特定应用，但仍可作为泌尿外科应用的候选材料进行进一步评估。
