Department of Surgery, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland.
School of Engineering, Institute for Bioengineering, University of Edinburgh, Edinburgh, UK.
Adv Exp Med Biol. 2021;1345:119-128. doi: 10.1007/978-3-030-82735-9_10.
Surgical repair for the end stage bladder disease utilises vascularised, autogenous and mucus-secreting gastrointestinal tissue to replace the diseased organ or to augment inadequate bladder tissue. Post-operatively, the compliance of the bowel is often enough to restore the basic shape, structure and function of the urinary bladder; however, lifelong post-operative complications are common. Comorbidities that result from interposition of intestinal tissue are metabolic and/or neuromechanical, and their incidence approaches 100%. The debilitating comorbidities and complications associated with such urological procedures may be mitigated by the availability of alternative, tissue-engineered, animal-derived extracellular matrix (ECM) scaffolds such as porcine urinary bladder matrix (UBM). Porcine UBM is a decellularized biocompatible, biodegradable biomaterial derived from the porcine urinary bladder. This chapter aims to describe the production and preparation techniques for porcine UBM for urinary bladder regenerative purposes.
外科手术修复终末期膀胱疾病时，利用血管化的、自体的和分泌黏液的胃肠道组织来替代患病器官或增强功能不足的膀胱组织。手术后，肠的顺应性通常足以恢复膀胱的基本形状、结构和功能；然而，术后长期并发症很常见。肠组织插入引起的合并症是代谢性和/或神经机械性的，其发生率接近 100%。通过使用替代的、组织工程的、动物衍生的细胞外基质 (ECM) 支架，如猪膀胱基质 (UBM)，可以减轻与这些泌尿外科手术相关的衰弱合并症和并发症。猪 UBM 是一种去细胞化的生物相容性、可生物降解的生物材料，来源于猪的膀胱。本章旨在描述用于膀胱再生目的的猪 UBM 的生产和准备技术。
