Halfter Norbert, Espinosa-Cano Eva, Pontes-Quero Gloria María, Ramírez-Jiménez Rosa Ana, Heinemann Christiane, Möller Stephanie, Schnabelrauch Matthias, Wiesmann Hans-Peter, Hintze Vera, Aguilar Maria Rosa
Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universität Dresden, Budapester Straße 27, 01069 Dresden, Germany.
Group of Biomaterials, Institute of Polymer Science and Technology ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, Spain.
J Funct Biomater. 2023 Mar 17;14(3):160. doi: 10.3390/jfb14030160.
Current limitations of wound dressings for treating chronic wounds require the development of novel approaches. One of these is the immune-centered approach, which aims to restore the pro-regenerative and anti-inflammatory properties of macrophages. Under inflammatory conditions, ketoprofen nanoparticles (KT NPs) can reduce pro-inflammatory markers of macrophages and increase anti-inflammatory cytokines. To assess their suitability as part of wound dressings, these NPs were combined with hyaluronan (HA)/collagen-based hydro- (HGs) and cryogels (CGs). Different HA and NP concentrations and loading techniques for NP incorporation were used. The NP release, gel morphology, and mechanical properties were studied. Generally, colonialization of the gels with macrophages resulted in high cell viability and proliferation. Furthermore, direct contact of the NPs to the cells reduced the level of nitric oxide (NO). The formation of multinucleated cells on the gels was low and further decreased by the NPs. For the HGs that produced the highest reduction in NO, extended ELISA studies showed reduced levels of the pro-inflammatory markers PGE2, IL-12 p40, TNF-α, and IL-6. Thus, HA/collagen-based gels containing KT NPs may represent a novel therapeutic approach for treating chronic wounds. Whether effects observed in vitro translate into a favorable profile on skin regeneration in vivo will require rigorous testing.
目前用于治疗慢性伤口的伤口敷料存在局限性，这就需要开发新的方法。其中一种是以免疫为中心的方法，其目的是恢复巨噬细胞的促再生和抗炎特性。在炎症条件下，酮洛芬纳米颗粒（KT NPs）可以降低巨噬细胞的促炎标志物水平，并增加抗炎细胞因子。为了评估它们作为伤口敷料一部分的适用性，将这些纳米颗粒与透明质酸（HA）/胶原蛋白基水凝胶（HGs）和冷冻凝胶（CGs）相结合。使用了不同的HA和纳米颗粒浓度以及纳米颗粒掺入的加载技术。研究了纳米颗粒的释放、凝胶形态和机械性能。一般来说，巨噬细胞在凝胶上的定植导致了高细胞活力和增殖。此外，纳米颗粒与细胞的直接接触降低了一氧化氮（NO）的水平。凝胶上多核细胞的形成较少，并且纳米颗粒进一步降低了其形成。对于产生最大程度NO减少的HGs，扩展酶联免疫吸附测定（ELISA）研究表明促炎标志物前列腺素E2（PGE2）、白细胞介素12 p40（IL-12 p40）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的水平降低。因此，含有KT NPs的HA/胶原蛋白基凝胶可能代表一种治疗慢性伤口的新方法。体外观察到的效果是否能转化为体内皮肤再生的良好表现，还需要进行严格的测试。
