Center of Excellence on Oral Microbiology and Immunology, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Oral Surgery, Faculty of Dentistry, Naresuan University, Phitsanulok 65000, Thailand.
Mar Drugs. 2023 Mar 12;21(3):175. doi: 10.3390/md21030175.
The appropriate storage protocol is one of the main limitations of translating tissue engineering technology to commercialized clinical applications. Recently, the development of a chitosan-derived composite scaffold incorporated with bioactive molecules has been reported as an excellent material to repair a critical size bony defect in mice calvaria. This study aims to determine the storage time and appropriate storage temperature of Chitosan/Biphasic Calcium Phosphate/Trichostatin A composite scaffold (CS/BCP/TSA scaffold) in vitro. The mechanical properties and in vitro bioactivity of trichostatin A (TSA) released from CS/BCP/TSA scaffolds in different storage times and temperatures were evaluated. Different storage times (0, 14, and 28 days) and temperatures (-18, 4, and 25 °C) did not affect the porosity, compressive strength, shape memory, and amount of TSA released. However, scaffolds stored at 25 °C and 4 °C were found to lose their bioactivity after 3- and 7-day storage periods, respectively. Thus, the CS/BCP/TSA scaffold should be stored in freezing conditions to preserve the long-term stability of TSA.
合适的储存方案是将组织工程技术转化为商业化临床应用的主要限制因素之一。最近，有研究报道了一种壳聚糖衍生的复合支架，其中掺入了生物活性分子，该材料被认为是修复小鼠颅骨临界尺寸骨缺损的优秀材料。本研究旨在确定壳聚糖/双相磷酸钙/曲古抑菌素 A 复合支架（CS/BCP/TSA 支架）在体外的储存时间和适宜的储存温度。评估了不同储存时间（0、14 和 28 天）和温度（-18、4 和 25°C）下 CS/BCP/TSA 支架中释放的曲古抑菌素 A（TSA）的机械性能和体外生物活性。不同的储存时间（0、14 和 28 天）和温度（-18、4 和 25°C）并不影响支架的孔隙率、压缩强度、形状记忆和 TSA 的释放量。然而，在 25°C 和 4°C 下储存的支架在分别储存 3 天和 7 天后，其生物活性丧失。因此，CS/BCP/TSA 支架应储存在冷冻条件下，以保持 TSA 的长期稳定性。
