Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Companion Animal Cancer Research Unit, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Vet Res Commun. 2023 Sep;47(3):1505-1521. doi: 10.1007/s11259-023-10106-0. Epub 2023 Mar 28.
Pulmonary hypertension (PH), an increase in pulmonary arterial pressure (PAP), may occur in dogs affected with myxomatous mitral valve disease (MMVD). Recent studies suggest that an accumulation of perivascular inflammatory cells may be involved with medial thickening which is a sign of the pulmonary artery remodelling in PH. The aim of this study was to characterise perivascular inflammatory cells in the surrounding pulmonary arteries of dogs with PH due to MMVD compared to MMVD dogs and healthy control dogs. Nineteen lung samples were collected from cadavers of small-breed dogs (control n = 5; MMVD n = 7; MMVD + PH n = 7). Toluidine blue stain and multiple IHC targeting α-SMA, vWF, CD20, CD68 and CD3 was performed to examine intimal and medial thickening, assess muscularisation of the small pulmonary arteries and characterise perivascular leucocytes. Medial thickening without intimal thickening of pulmonary arteries and muscularisation of normally non-muscularised small pulmonary arteries was observed in the MMVD and MMVD + PH groups compared with the control group. The perivascular numbers of B lymphocytes, T lymphocytes and macrophages was significantly increased in the MMVD + PH group compared with the MMVD and control groups. In contrast, the perivascular number of mast cells was significantly higher in the MMVD group compared with the MMVD + PH and control groups. This study suggested that pulmonary artery remodelling as medial thickening and muscularisation of the normally non-muscular small pulmonary arteries is accompanied by the accumulation of perivascular inflammatory cells.
肺动脉高压（PH）是指肺动脉压力（PAP）升高，可能发生在患有黏液瘤性二尖瓣疾病（MMVD）的犬中。最近的研究表明，血管周围炎症细胞的积累可能与中膜增厚有关，中膜增厚是 PH 中肺动脉重塑的标志。本研究旨在比较 MMVD 伴 PH 犬与 MMVD 犬和健康对照犬的 PH 犬周围肺小动脉中血管周围炎症细胞的特征。从小型犬尸体中收集了 19 个肺样本（对照组 n=5；MMVD 组 n=7；MMVD+PH 组 n=7）。使用甲苯胺蓝染色和针对α-SMA、vWF、CD20、CD68 和 CD3 的多重免疫组化染色，检查内膜和中膜增厚，评估小肺动脉的肌化，并对血管周围白细胞进行特征描述。与对照组相比，MMVD 组和 MMVD+PH 组的肺小动脉出现中膜增厚而无内膜增厚，且正常非肌化的小肺动脉发生肌化。与 MMVD 组和对照组相比，MMVD+PH 组的血管周围 B 淋巴细胞、T 淋巴细胞和巨噬细胞数量明显增加。相比之下，MMVD 组的血管周围肥大细胞数量明显高于 MMVD+PH 组和对照组。本研究表明，肺小动脉重塑表现为中膜增厚和正常非肌化小肺动脉的肌化，同时伴有血管周围炎症细胞的积累。
