[ArdB蛋白对EcoAI核酸内切酶的抗限制活性]

[Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease].

作者信息

Kudryavtseva A A, Alekhin V A, Lebedeva M D, Cséfalvay E, Weiserova M, Manukhov I V

机构信息

Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow oblast, 141707 Russia.

Autonomous non-profit educational organization "Phystech Lyceum" named after P.L. Kapitsa, Dolgoprudny, Moscow oblast, 141707 Russia.

出版信息

Mol Biol (Mosk). 2023 Jan-Feb;57(1):101-105. doi: 10.31857/S0026898423010056.

DOI:10.31857/S0026898423010056

PMID:36976744

Abstract

ArdB proteins are known to inhibit the activity of the type I restriction-modification (RM-I) system, in particular EcoKI (IA family). The mechanism of ArdB's activity still remains unknown; the spectrum of targets inhibited has been poorly studied. In this work, it was shown that the presence of the ardB gene from the R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. Due to the absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be assumed that the mechanism of the anti-restriction activity of this protein does not depend on the sequence DNA at the recognition site nor the structure of the restriction enzyme of the RM-I systems.

摘要

已知ArdB蛋白可抑制I型限制修饰（RM-I）系统的活性，特别是EcoKI（IA家族）。ArdB的活性机制仍然未知；对其抑制的靶标谱研究甚少。在这项研究中，结果表明，R64质粒中的ardB基因的存在可抑制大肠杆菌TG1细胞中EcoAI核酸内切酶（IB家族）的活性。由于ArdB对特定RM-I系统缺乏特异性（它可抑制IA和IB家族），可以推测该蛋白的抗限制活性机制不依赖于识别位点的DNA序列，也不依赖于RM-I系统限制酶的结构。