Becker Anne A M J, Munden Stacie, McCabe Evonne, Hurley Daniel, Fanning Séamus, Chapwanya Aspinas, Butaye Patrick
Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
Science Center South, School of Public Health, Physiotherapy & Sports Science, University College Dublin Belfield, D04 N2E5 Dublin, Ireland.
Vet Sci. 2023 Mar 10;10(3):215. doi: 10.3390/vetsci10030215.
Endometritis is one of the most important causes of infertility in dairy cows, resulting in high economic losses in the dairy industry. Though the presence of a commensal uterine microbiota is now well established, the complex role of these bacteria in genital health, fertility, and susceptibility to uterine diseases remains unclear. In this study, we explore the endometrial microbiota through 16S rRNA gene profiling from cytobrush samples taken ex vivo from healthy, pregnant, and endometritis cows. There were no significant differences between healthy and pregnant cows, whose uterine microbiota were dominated by , , , and . Compared to pregnant and clinically healthy cows, the uterine bacterial community of endometritis cows was significantly decreased in species diversity ( < 0.05), reflecting uneven community composition in different patterns with either dominance of , , and or Actinobacteria.
子宫内膜炎是奶牛不孕症的最重要原因之一，给乳制品行业造成了巨大的经济损失。尽管共生子宫微生物群的存在现已得到充分证实，但这些细菌在生殖健康、生育能力和子宫疾病易感性方面的复杂作用仍不清楚。在本研究中，我们通过对从健康、怀孕和患有子宫内膜炎的奶牛离体采集的细胞刷样本进行16S rRNA基因谱分析，来探索子宫内膜微生物群。健康奶牛和怀孕奶牛之间没有显著差异，它们的子宫微生物群主要由 、 、 、 和 组成。与怀孕奶牛和临床健康奶牛相比，子宫内膜炎奶牛的子宫细菌群落物种多样性显著降低（ < 0.05），反映出不同模式下群落组成不均衡，要么以 、 、 和 为主，要么以放线菌为主。
