基质富含半胱氨酸蛋白，一种来自的类星系蛋白，在体外诱导球霰石形成。
The Matrix Protein Cysrichin, a Galaxin-like Protein from , Induces Vaterite Formation In Vitro.
作者信息
Xia Zhonghui, Zhang Xin, Zhou Yujuan, Yao Liping, Zhang Zhen, Zhang Rongqing, Liu Xiaojun
机构信息
China National Biotec Company Limited, Beijing 100191, China.
Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314000, China.
出版信息
Biology (Basel). 2023 Mar 15;12(3):447. doi: 10.3390/biology12030447.
In this study, we cloned a novel matrix protein, cysrichin, with 16.03% homology and a similar protein structure to the coral biomineralized protein galaxin. Tissue expression analysis showed that cysrichin was mainly expressed in mantle and gill tissues. In situ hybridization indicated that cysrichin mRNA was detected in the entire epithelium region of mantle tissue. RNAi analysis and shell notching experiment confirmed that cysrichin participates in the prismatic layer and nacreous layer formation of the shell. An in vitro crystallization experiment showed that the cysrichin protein induced lotus-shaped and round-shaped crystals, which were identified as vaterite crystals. These results may provide new clues for understanding the formation of vaterite in freshwater shellfish.
在本研究中，我们克隆了一种新型基质蛋白——富含半胱氨酸蛋白（cysrichin），其与珊瑚生物矿化蛋白 galaxin 具有 16.03%的同源性且蛋白质结构相似。组织表达分析表明，富含半胱氨酸蛋白主要在套膜和鳃组织中表达。原位杂交表明，在套膜组织的整个上皮区域检测到富含半胱氨酸蛋白的 mRNA。RNA 干扰分析和贝壳刻痕实验证实，富含半胱氨酸蛋白参与贝壳棱柱层和珍珠层的形成。体外结晶实验表明，富含半胱氨酸蛋白诱导形成莲花形和圆形晶体，鉴定为球霰石晶体。这些结果可能为理解淡水贝类中球霰石的形成提供新线索。
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