液体活检在早期子宫内膜癌手术中循环肿瘤细胞检测的初步研究。
Circulating Tumor Cell Detection by Liquid Biopsy during Early-Stage Endometrial Cancer Surgery: A Pilot Study.
机构信息
Department of Gynecological and Breast Surgery, Montpellier University Hospital, 34000 Montpellier, France.
Clinical Research and Epidemiology Unit, Montpellier University Hospital, 34000 Montpellier, France.
出版信息
Biomolecules. 2023 Feb 24;13(3):428. doi: 10.3390/biom13030428.
The recurrence of non-metastatic endometrial carcinoma (EC) (6 to 21%) might be due to disseminated tumor cells. This feasibility study investigated whether circulating tumor cells (CTCs) were detectable in blood samples from the peripheral and ovarian veins of 10 patients undergoing laparoscopic resection of stage I-II EC between July 2019 and September 2021. CTCs were detected using the CellSearch system (i) preoperatively (T0) in peripheral blood, (ii) after ovary suspensory ligament pediculation in ovarian vein blood (T1), and (iii) before colpotomy in peripheral blood (T2). CTCs were detected only in ovarian vein samples in 8/10 patients. The CTC median number did not differ with patient age (37 (min-max: 0-91) in <70-year-old vs. 11 (0-65) in ≥70 year-old women, = 0.59), tumor grade (15 (0-72) for grade 1 vs. 15 (0-91) for grade 2, = 0.97), FIGO stage (72 (27-91) vs. 2 (0-65) vs. 3 (0-6]) for stage IA, B, and II, respectively; = 0.08), and tumor size (40 (2-72) for size < 30 mm vs. 4 (0-91) for size ≥ 30 mm, = 0.39). Estrogen receptor-positive CTCs and CTC clusters were identified. The prognostic and therapeutic values of CTCs released during EC surgery need to be determined.
非转移性子宫内膜癌（EC）（6-21%）的复发可能是由于播散的肿瘤细胞。本可行性研究调查了 10 名在 2019 年 7 月至 2021 年 9 月期间接受腹腔镜 I 期-II 期 EC 切除术的患者的外周静脉和卵巢静脉血液样本中是否可检测到循环肿瘤细胞（CTC）。使用 CellSearch 系统检测 CTC（i）术前（T0）外周血，（ii）卵巢悬韧带蒂部结扎后卵巢静脉血（T1），（iii）剖腹术前行（T2）外周血。在 10 名患者中的 8 名中仅在卵巢静脉样本中检测到 CTC。CTC 的中位数数量与患者年龄无关（<70 岁患者的 37（最小-最大：0-91）与≥70 岁患者的 11（0-65）， = 0.59），肿瘤分级（1 级的 15（0-72）与 2 级的 15（0-91）， = 0.97），FIGO 分期（IA、B 和 II 期分别为 72（27-91）、2（0-65）和 3（0-6]， = 0.08），肿瘤大小（<30mm 的 40（2-72）与≥30mm 的 4（0-91）， = 0.39）。鉴定出雌激素受体阳性的 CTC 和 CTC 簇。需要确定在 EC 手术期间释放的 CTC 的预后和治疗价值。
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