Department of Mechanical Engineering, Politecnico di Milano, 20156 Milan, Italy.
Biosensors (Basel). 2023 Mar 9;13(3):362. doi: 10.3390/bios13030362.
The extensive use of antibiotics has become a serious concern due to certain deficiencies in wastewater facilities, their resistance to removal, and their toxic effects on the natural environment. Therefore, substantial attention has been given to the detection of antibiotics because of their potential detriment to the ecosystem and human health. In the present study, a novel design of indium tin oxide (ITO) coated U-shaped fiber optic lossy mode resonance (LMR) biosensor is presented for the sensitive detection of the antibiotic ciprofloxacin (CIP). The performance of the designed U-shaped LMR sensor is characterized in terms of its sensitivity, full width at half maximum (FWHM), the figure of merit (FOM), and the limit of detection (LOD). For the proposed U-shaped LMR sensing probe, the various crucial factors such as the thickness (d) of the ITO layer, sensing region length (L), and bending radius (R) are optimized. The thickness of the ITO layer is optimized in such a way that two LMR curves are observed in the transmission spectrum and, thereafter, the performance parameters are evaluated for each LMR. It is observed that the designed U-shaped LMR sensor with optimized parameters shows an approximately seven-fold enhancement in sensitivity compared to the straight-core fiber optic LMR sensor. The numerical results revealed that the designed U-shaped fiber optic LMR biosensor can provide a maximum sensitivity of 17,209.9 nm/RIU with the highest FOM of 91.42 RIU, and LOD of 6.3 × 10 RIU for the detection of CIP hydrochloride in the concentration range of 0.001 to 0.029 mol∙dm. Thus, it is believed that the designed LMR biosensor can practically explore its potential use in environmental monitoring and biomedical applications and hence, opens a new window of opportunity for the researchers working in the field of U-shaped fiber optic LMR biosensing.
由于废水设施的某些缺陷、抗生素的抗去除性以及它们对自然环境的毒性影响，抗生素的广泛使用已成为一个严重的问题。因此，人们对抗生素的检测给予了极大的关注，因为它们可能对生态系统和人类健康造成危害。在本研究中，提出了一种新型的氧化铟锡（ITO）涂覆的 U 型光纤损耗模式共振（LMR）生物传感器设计，用于灵敏检测抗生素环丙沙星（CIP）。所设计的 U 型 LMR 传感器的性能是根据其灵敏度、半峰全宽（FWHM）、品质因数（FOM）和检测限（LOD）来表征的。对于所提出的 U 型 LMR 传感探头，优化了各种关键因素，如 ITO 层的厚度（d）、传感区长度（L）和弯曲半径（R）。ITO 层的厚度进行了优化，使得在透射光谱中观察到两个 LMR 曲线，然后评估每个 LMR 的性能参数。结果表明，与直芯光纤 LMR 传感器相比，优化参数后的设计 U 型 LMR 传感器的灵敏度提高了约七倍。数值结果表明，设计的 U 型光纤 LMR 生物传感器可以提供最大灵敏度为 17,209.9nm/RIU，最高 FOM 为 91.42RIU，以及检测 CIP 盐酸盐的 LOD 为 6.3×10 RIU，检测浓度范围为 0.001 至 0.029 mol·dm。因此，人们相信设计的 LMR 生物传感器可以在环境监测和生物医学应用中实际探索其潜在用途，从而为从事 U 型光纤 LMR 生物传感领域的研究人员开辟了新的机会。
