Department of Zoology, Maharshi Dayanand University, Rohtak 124001, India.
Department of Biochemistry, Maharshi Dayanand University, Rohtak 124001, India.
Biosensors (Basel). 2023 Mar 15;13(3):386. doi: 10.3390/bios13030386.
An electrochemical biosensor was fabricated using nanoparticles of acetylcholinesterase (AChE) and choline oxidase (ChO)/Pt nanoparticles (PtNPs)/porous graphene oxide nanosheet (GONS) composite. A pencil graphite electrode (PGE) was used for the electrodeposition of nanocomposite and the determination of acetylcholine (ACh), a neurotransmitter. Various techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectra and cyclic voltammetry (CV) were used for characterization. This biosensor (AChENPs-ChONPs/GONS/PtNPs/PGE) indicated a very short response time (3 s), a lower limit of detection (0.001 µM), good linearity (0.001-200 µM), longer storage stability (6 months) and better reproducibility. The percent analytical recoveries of added acetylcholine in serum (5.0 and 10 µM) were found to be 97.6 ± 0.7 and 96.5 ± 0.3 for the present biosensor. The coefficients of variation were obtained to be 8% and 3.25%, correspondingly. The biosensor was applied to measure the ACh amount in the serum of healthy individuals and patients with Alzheimer's disease. The number of interferents had no effect on the biosensor at their physiological concentrations.
采用乙酰胆碱酯酶（AChE）纳米粒子和胆碱氧化酶（ChO）/Pt 纳米粒子（PtNPs）/多孔氧化石墨烯纳米片（GONS）复合材料制备了电化学生物传感器。使用铅笔石墨电极（PGE）进行纳米复合材料的电沉积，并测定神经递质乙酰胆碱（ACh）。采用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X 射线衍射（XRD）、傅里叶变换红外（FTIR）光谱和循环伏安法（CV）等多种技术进行了表征。该生物传感器（AChENPs-ChONPs/GONS/PtNPs/PGE）具有非常短的响应时间（3 s）、较低的检测限（0.001 µM）、良好的线性范围（0.001-200 µM）、更长的储存稳定性（6 个月）和更好的重现性。在血清中加入的乙酰胆碱（5.0 和 10 µM）的分析回收率分别为 97.6 ± 0.7%和 96.5 ± 0.3%。相应的变异系数分别为 8%和 3.25%。该生物传感器用于测量健康个体和阿尔茨海默病患者血清中的 ACh 含量。在生理浓度下，干扰物的数量对生物传感器没有影响。
