Facultad de Informática, Universidad Autónoma de Querétaro, Querétaro 76010, Mexico.
División de Investigación, Hospital Juárez de México, Ciudad de México 07760, Mexico.
Cells. 2023 Mar 8;12(6):834. doi: 10.3390/cells12060834.
Triple-negative breast cancer (TNBC) is one of the most aggressive subtypes of breast cancer and has the worst prognosis. In patients with TNBC tumors, the tumor cells have been reported to have mesenchymal features, which help them migrate and invade. Various studies on cancer have revealed the importance of microRNAs (miRNAs) in different biological processes of the cell in that aberrations, in their expression, lead to alterations and deregulations in said processes, giving rise to tumor progression and aggression. In the present work, we determined the miRNAs that are deregulated in the epithelial-mesenchymal transition process in breast cancer. We discovered that 25 miRNAs that regulate mesenchymal genes are overexpressed in patients with TNBC. We found that miRNA targets modulate different processes and pathways, such as apoptosis, FoxO signaling pathways, and Hippo. We also found that the expression level of miR-934 is specific to the molecular subtype of the triple-negative breast cancer and modulates a set of related epithelial-mesenchymal genes. We determined that miR-934 inhibition in TNBC cell lines inhibits the migratory abilities of tumor cells.
三阴性乳腺癌（TNBC）是乳腺癌中侵袭性最强的亚型之一，预后最差。在 TNBC 肿瘤患者中，据报道肿瘤细胞具有间充质特征，这有助于它们迁移和侵袭。各种癌症研究揭示了 microRNAs（miRNAs）在细胞不同生物学过程中的重要性，因为它们的表达异常会导致这些过程发生改变和失调，从而导致肿瘤的进展和侵袭。在本工作中，我们确定了在乳腺癌上皮-间充质转化过程中失调的 miRNAs。我们发现，在 TNBC 患者中，有 25 个调节间充质基因的 miRNAs 过表达。我们发现 miRNA 靶标调节不同的过程和途径，如细胞凋亡、FoxO 信号通路和 Hippo。我们还发现，miR-934 的表达水平是三阴性乳腺癌分子亚型特有的，调节了一组相关的上皮-间充质基因。我们确定在 TNBC 细胞系中抑制 miR-934 抑制肿瘤细胞的迁移能力。
