Kinutani M, Coltey M, Le Douarin N M
Cell. 1986 Apr 25;45(2):307-14. doi: 10.1016/0092-8674(86)90395-8.
Xenogeneic spinal cord chimeras were constructed by grafting fragments of quail neural primordium into chick embryos at 2 days of incubation. Hatched birds displayed normal motor behavior for about 5 to 7 weeks, whereupon they developed a neurological syndrome; in the grafted spinal cord the pathological signs of the disease were very similar to those of the active plaques of multiple sclerosis and of the lesions of experimental allergic encephalomyelitis and neuritis, including Ia expression by brain capillary endothelia, rupture of the blood-brain barrier, leukocytic infiltration in the nervous tissue, and demyelination. In the animals at the most advanced stage of the disease an autoimmune attack occurred on the host's nervous system with the same histopathological signs.
通过在孵化2天时将鹌鹑神经原基片段移植到鸡胚中构建了异种脊髓嵌合体。孵化出的鸟类在大约5至7周内表现出正常的运动行为，随后出现神经综合征；在移植的脊髓中，疾病的病理体征与多发性硬化症的活动性斑块以及实验性变应性脑脊髓炎和神经炎的病变非常相似，包括脑毛细血管内皮细胞表达Ia、血脑屏障破裂、神经组织中的白细胞浸润和脱髓鞘。在疾病最晚期的动物中，宿主神经系统发生了具有相同组织病理学体征的自身免疫攻击。
