Jing Lunan, Xue Jingqi, Jiang Xin, Xiao Naiyong, Pan Hao, Li Jiarou, Wang Dajun, Jiang Qingqing, Shi Wenzheng
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Yantai Haiyu Foodstuffs Co., Ltd. (Yantai), Yantai 264000, China.
Foods. 2023 Mar 11;12(6):1184. doi: 10.3390/foods12061184.
The evolution of quality attributes and their association with the protein properties of lightly tilapias fillets salted with different replacement proportions of NaCl with KCl (0%, 10%, 30%, 50%, 70%, 100%) at the same ionic strength were investigated. KCl replacements using optimal substitution (50% of KCl) contributed to maintaining desired quality properties. Further, KCl replacement (about 50~70% of KCl) led to the insolubilization and weakened stability of myofibrillar proteins, represented by the unfolding of the myofibrillar protein, increased surface hydrophilic points, and strengthened internal protein-protein interaction, resulting in the structurally reinforced hardness and lower water-holding capacity. Excessive replacement (more than 70% of KCl) showed apparent deterioration in taste quality, coloration, and hardness received by sensory sensation caused by immoderate hydrolysis and aggravated oxidation of the myofibrillar protein. In this sense, insights into KCl replacements on protein properties might be a positive approach to improving quality attributes of lightly salted tilapias fillets.
研究了在相同离子强度下,用不同比例(0%、10%、30%、50%、70%、100%)的KCl替代NaCl对罗非鱼鱼片轻度腌制后的品质属性及其与蛋白质特性之间关系的影响。采用最佳替代比例(50%的KCl)进行KCl替代有助于保持所需的品质特性。此外,KCl替代(约50%~70%的KCl)会导致肌原纤维蛋白的不溶性增加和稳定性减弱,表现为肌原纤维蛋白的展开、表面亲水性点增加以及内部蛋白质-蛋白质相互作用增强,从而导致结构强化的硬度和较低的持水能力。过量替代(超过70%的KCl)会导致口感质量、色泽和硬度明显下降,这是由于肌原纤维蛋白过度水解和氧化加剧,通过感官感受表现出来。从这个意义上说,了解KCl替代对蛋白质特性的影响可能是改善轻度腌制罗非鱼鱼片品质属性的一种有效方法。