Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín (CSIC), Profesor Albareda N° 1, 18008 Granada, Spain.
Departmento de Biología Ambiental, Facultad de Ciencias, Universidad de Navarra, Irunlarrea No 1, 31008 Pamplona, Spain.
Int J Mol Sci. 2023 Mar 8;24(6):5193. doi: 10.3390/ijms24065193.
Climate change is leading to combined drought and high temperature stress in many areas, drastically reducing crop production, especially for high-water-consuming crops such as maize. This study aimed to determine how the co-inoculation of an arbuscular mycorrhizal (AM) fungus () and the PGPR (Bm) alters the radial water movement and physiology in maize plants in order to cope with combined drought and high temperature stress. Thus, maize plants were kept uninoculated or inoculated with (AM), with (Bm) or with both microorganisms (AM + Bm) and subjected or not to combined drought and high temperature stress (D + T). We measured plant physiological responses, root hydraulic parameters, aquaporin gene expression and protein abundances and sap hormonal content. The results showed that dual AM + Bm inoculation was more effective against combined D + T stress than single inoculation. This was related to a synergistic enhancement of efficiency of the phytosystem II, stomatal conductance and photosynthetic activity. Moreover, dually inoculated plants maintained higher root hydraulic conductivity, which was related to regulation of the aquaporins , , and and levels of plant sap hormones. This study demonstrates the usefulness of combining beneficial soil microorganisms to improve crop productivity under the current climate-change scenario.
气候变化导致许多地区出现干旱和高温胁迫相结合的情况，极大地减少了作物的产量，特别是对玉米等高耗水作物。本研究旨在确定丛枝菌根（AM）真菌（）和植物根际促生菌（PGPR）（Bm）的共接种如何改变玉米植株的径向水分运动和生理机能，以应对干旱和高温胁迫相结合的情况。因此，将玉米植株保持未接种或接种 AM、Bm 或两种微生物（AM + Bm），并进行或不进行干旱和高温胁迫相结合（D + T）。我们测量了植物生理响应、根系水力参数、水通道蛋白基因表达和蛋白丰度以及汁液激素含量。结果表明，与单独接种相比，双重 AM + Bm 接种对 D + T 胁迫更有效。这与植物系统 II、气孔导度和光合作用效率的协同增强有关。此外，双重接种的植物保持较高的根系水力传导性，这与水通道蛋白、、和的调节以及植物汁液激素水平有关。本研究证明了在当前气候变化情景下，结合有益土壤微生物来提高作物生产力的有效性。
