State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, Qingdao 266237, China.
National Glycoengineering Research Center, Shandong University, Binhai Road 72, Qingdao 266237, China.
Int J Mol Sci. 2023 Mar 17;24(6):5759. doi: 10.3390/ijms24065759.
A large-scale application of sophorolipids (SLs) was blocked by their high production cost. One feasible way to reduce the cost of SL production is to develop cheap feedstocks as the substrates for SL fermentation. In the present work, cottonseed molasses (CM), a waste from raffinose production, was used as the hydrophilic substrate;, and cottonseed oil (CO) was used as a hydrophobic substrate for SL production by CGMCC 1576. The primary optimization of carbon sources, nitrogen source and inorganic salts, produced 57.6 ± 2.3 g/L of total SLs and 24.0 ± 1.2 g/L of lactonic SLs on CM and CO, almost equal to the titer of SLs produced from glucose and oleic. A response surface method was applied to optimize the fermentation medium for growth and SL production of . The production of total SLs reached 58.4 ± 3.4 g/L, and lactonic SLs were elevated to more than 25.0 ± 1.9 g/L. HPLC-MS analysis showed that the compositions of SLs produced by on CM and CO were very similar to those on glucose and oleic acid. These results suggested that cottonseed molasses and cottonseed oil can be used as renewable cheap substrates for the reduced-cost production of SLs.
棉子糖蜜和棉籽油可作为可再生廉价底物用于低成本发酵生产表面活性剂
大规模应用槐糖脂（SLs）受到其高生产成本的限制。降低 SL 生产成本的一种可行方法是开发廉价的原料作为 SL 发酵的底物。本研究以棉子糖生产副产物棉子糖蜜（CM）为亲水性底物，棉籽油（CO）为疏水性底物，由 CGMCC 1576 发酵生产 SLs。通过对碳源、氮源和无机盐的初步优化，CM 和 CO 上总 SLs 和内酯型 SLs 的产量分别达到 57.6 ± 2.3 g/L 和 24.0 ± 1.2 g/L，与葡萄糖和油酸生产的 SLs 产量相当。采用响应面法对 发酵生长和 SL 生产的培养基进行了优化。总 SLs 的产量达到 58.4 ± 3.4 g/L，内酯型 SLs 提高到 25.0 ± 1.9 g/L 以上。HPLC-MS 分析表明， 利用 CM 和 CO 生产的 SLs 组成与葡萄糖和油酸非常相似。这些结果表明，棉子糖蜜和棉籽油可作为可再生廉价底物，用于降低成本生产 SLs。
