NanoTechnas-Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko St. 24, LT-03225 Vilnius, Lithuania.
Institute of Chemistry, Vilnius University, Naugarduko St. 24, LT-03225 Vilnius, Lithuania.
Int J Mol Sci. 2023 Mar 18;24(6):5803. doi: 10.3390/ijms24065803.
In this study, ZnO nanostructures with different types of morphologies and particle sizes were evaluated and applied for the development of an immunosensor. The first material was composed of spherical, polydisperse nanostructures with a particle size in the range of 10-160 nm. The second was made up of more compact rod-like spherical nanostructures with the diameter of these rods in the range of 50-400 nm, and approximately 98% of the particles were in the range of 20-70 nm. The last sample of ZnO was made up of rod-shaped particles with a diameter of 10-80 nm. These ZnO nanostructures were mixed with Nafion solution and drop-casted onto screen-printed carbon electrodes (SPCE), followed by a further immobilization of the prostate-specific antigen (PSA). The affinity interaction of PSA with monoclonal antibodies against PSA (anti-PSA) was evaluated using the differential pulse voltammetry technique. The limit of detection and limit of quantification of anti-PSA were determined as 1.35 nM and 4.08 nM for compact rod-shaped spherical ZnO nanostructures, and 2.36 nM and 7.15 nM for rod-shaped ZnO nanostructures, respectively.
在这项研究中，评估了具有不同形貌和粒径的 ZnO 纳米结构，并将其应用于免疫传感器的开发。第一种材料由粒径在 10-160nm 范围内的球形、多分散纳米结构组成。第二种是由更紧密的棒状球形纳米结构组成，这些棒的直径在 50-400nm 范围内，约 98%的颗粒在 20-70nm 范围内。最后一种 ZnO 样品由直径为 10-80nm 的棒状颗粒组成。这些 ZnO 纳米结构与 Nafion 溶液混合，滴铸到丝网印刷碳电极（SPCE）上，然后进一步固定前列腺特异性抗原（PSA）。使用差分脉冲伏安法技术评估 PSA 与针对 PSA 的单克隆抗体（抗 PSA）的亲和相互作用。对于紧密棒状球形 ZnO 纳米结构，抗 PSA 的检测限和定量限分别为 1.35nM 和 4.08nM，对于棒状 ZnO 纳米结构，检测限和定量限分别为 2.36nM 和 7.15nM。
