Department of Gynecology and Obstetrics, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirão Preto, São Paulo 14049-900, Brazil.
Regional Blood Center, Medical School of Hemocenter Foundation of Ribeirão Preto, University of Sao Paulo, Ribeirão Preto, São Paulo 14051-140, Brazil.
Int J Mol Sci. 2023 Mar 22;24(6):5963. doi: 10.3390/ijms24065963.
Menstrual blood mesenchymal stem cells (MenSCs) have gained prominence in the endometriosis scientific community, given their multifunctional roles in regenerative medicine as a noninvasive source for future clinical applications. In addition, changes in post-transcriptional regulation via miRNAs have been explored in endometriotic MenSCs with a role in modulating proliferation, angiogenesis, differentiation, stemness, self-renewal, and the mesenchymal-epithelial transition process. In this sense, homeostasis of the miRNA biosynthesis pathway is essential for several cellular processes and is related to the self-renewal and differentiation of progenitor cells. However, no studies have investigated the miRNA biogenesis pathway in endometriotic MenSCs. In this study, we profiled the expression of eight central genes for the miRNA biosynthesis pathway under experimental conditions involving a two-dimensional culture of MenSCs obtained from healthy women ( = 10) and women with endometriosis ( = 10) using RT-qPCR and reported a two-fold decrease in expression in the disease. In addition, miR-128-3p, miR-27a-3p, miR-27b-3p, miR-181a-5p, miR-181b-5p, miR-452-3p, miR-216a-5p, miR-216b-5p, and miR-93-5p, which have been associated with endometriosis, were identified through in silico analyses as negative regulators of . Because DROSHA is essential for miRNA maturation, our findings may justify the identification of different profiles of miRNAs with DROSHA-dependent biogenesis in endometriosis.
经血间充质干细胞（MenSCs）因其在再生医学中的多功能作用而成为子宫内膜异位症科学界的关注焦点，作为未来临床应用的非侵入性来源。此外，通过 miRNA 进行的转录后调控变化在子宫内膜异位症 MenSCs 中得到了探索，其在调节增殖、血管生成、分化、干细胞特性、自我更新和间充质上皮转化过程中具有重要作用。从这个意义上说，miRNA 生物合成途径的动态平衡对于许多细胞过程至关重要，并且与祖细胞的自我更新和分化有关。然而，目前还没有研究调查子宫内膜异位症 MenSCs 中的 miRNA 生物发生途径。在这项研究中，我们使用 RT-qPCR 分析了来自健康女性（n=10）和子宫内膜异位症女性（n=10）的 MenSCs 的二维培养实验条件下 miRNA 生物合成途径的八个核心基因的表达谱，并报告在疾病中 表达降低了两倍。此外，通过计算分析，miR-128-3p、miR-27a-3p、miR-27b-3p、miR-181a-5p、miR-181b-5p、miR-452-3p、miR-216a-5p、miR-216b-5p 和 miR-93-5p 被鉴定为 的负调控因子，这些 miRNA 与子宫内膜异位症有关。由于 DROSHA 对 miRNA 成熟至关重要，我们的发现可能证明了在子宫内膜异位症中存在不同的 DROSHA 依赖性 miRNA 生物发生谱。
