Ochocińska Agnieszka, Wysocka-Mincewicz Marta, Świderska Jolanta, Cukrowska Bożena
Department of Biochemistry, Radioimmunology and Experimental Medicine, The Children's Memorial Health Institute, Aleja Dzieci Polskich 20, 04-730 Warsaw, Poland.
Clinic of Endocrinology and Diabetology, The Children's Memorial Health Institute, Aleja Dzieci Polskich 20, 04-730 Warsaw, Poland.
J Clin Med. 2023 Mar 9;12(6):2151. doi: 10.3390/jcm12062151.
Biochemical abnormalities in the course of type 1 diabetes (T1D) may cause the production/activation of various proteins and peptides influencing treatment and causing a risk of complications. The aim of this study was to assess concentrations of selected serum substances involved in the pathogenesis and course of T1D and to correlate their concentrations with the duration of T1D. The study included patients with T1D ( = 156) at the age of 3-17, who were divided according to the duration of the disease into those newly diagnosed ( = 30), diagnosed after 3-5 ( = 77), 6-7 ( = 25), and over 7 ( = 24) years from the onset of T1D, and age-matched healthy controls ( = 30). Concentrations of amylin (IAPP), proamylin (proIAPP), catestatin (CST), chromogranin A (ChgA), nerve growth factor (NFG), platelet-activating factor (PAF), uromodulin (UMOD), and intestinal fatty acid binding protein (I-FABP) were measured in sera using immunoenzymatic tests. There were significant differences in concentrations of all the substances except UMOD and NGF between T1D patients and healthy children. The duration of the disease affected concentrations of CST, ChgA, PAF, and NGF, i.e., proteins/peptides which could have an impact on the course of T1D and the development of complications. In long-term patients, a decrease in concentrations of CST and ChgA, and an increase in PAF concentrations were found. In the case of NGF, a decrease was observed after the initial high values, followed by an increase over 7 years after T1D diagnosis. Concluding, the results show that concentrations of selected serum indicators may change in the course of T1D. Further studies are needed to establish whether these indicators could be used in the context of predicting long-term complications.
1型糖尿病（T1D）病程中的生化异常可能导致多种蛋白质和肽的产生/激活，这些蛋白质和肽会影响治疗并引发并发症风险。本研究的目的是评估参与T1D发病机制和病程的特定血清物质的浓度，并将它们的浓度与T1D病程相关联。该研究纳入了3至17岁的T1D患者（n = 156），根据病程将其分为新诊断患者（n = 30）、发病后3至5年诊断的患者（n = 77）、6至7年诊断的患者（n = 25）以及发病超过7年诊断的患者（n = 24），并选取了年龄匹配的健康对照者（n = 30）。使用免疫酶法检测血清中胰淀素（IAPP）、前胰淀素（proIAPP）、抑制素（CST）、嗜铬粒蛋白A（ChgA）、神经生长因子（NFG）、血小板活化因子（PAF）、尿调节素（UMOD）和肠脂肪酸结合蛋白（I-FABP）的浓度。T1D患者与健康儿童之间，除UMOD和NGF外，所有物质的浓度均存在显著差异。病程影响了CST、ChgA、PAF和NGF的浓度，即这些蛋白质/肽可能会影响T1D的病程和并发症的发生发展。在病程较长的患者中，发现CST和ChgA浓度降低，PAF浓度升高。对于NGF，在最初的高值之后出现下降，然后在T1D诊断7年后又升高。总之，结果表明特定血清指标的浓度在T1D病程中可能会发生变化。需要进一步研究以确定这些指标是否可用于预测长期并发症。
