Orosz Ferenc
Research Centre for Natural Sciences, Institute of Enzymology, 1117 Budapest, Hungary.
J Fungi (Basel). 2023 Mar 19;9(3):376. doi: 10.3390/jof9030376.
The seven most early diverging lineages of the 18 phyla of fungi are the non-terrestrial fungi, which reproduce through motile flagellated zoospores. There are genes/proteins that are present only in organisms with flagellum or cilium. It was suggested that TPPP-like proteins (proteins containing at least one complete or partial p25alpha domain) are among them, and a correlation between the incidence of the p25alpha domain and the eukaryotic flagellum was hypothesized. Of the seven phyla of flagellated fungi, six have been known to contain TPPP-like proteins. Aphelidiomycota, one of the early-branching phyla, has some species (e.g., ) that retain the flagellum, whereas the genus has lost the flagellum. The first two Aphelidiomycota genomes ( and ) were sequenced and published last year. A BLASTP search revealed that does not have a TPPP, but , which possesses pseudocilium, does have a TPPP. This TPPP is the 'long-type' which occurs mostly in animals as well as other Opisthokonta. has a 'fungal-type' TPPP, which is found only in some flagellated fungi. These data on Aphelidiomycota TPPP proteins strengthen the correlation between the incidence of p25alpha domain-containing proteins and that of the eukaryotic flagellum/cilium.
真菌18个门中最早分化的7个谱系是非陆生真菌，它们通过具鞭毛的游动孢子进行繁殖。存在一些仅在具有鞭毛或纤毛的生物体中才有的基因/蛋白质。有人提出，类TPPP蛋白（至少含有一个完整或部分p25α结构域的蛋白质）属于此类，并推测p25α结构域的出现与真核生物鞭毛之间存在相关性。在7个有鞭毛的真菌门中，已知有6个门含有类TPPP蛋白。最早分支的门之一的无柄菌门有一些物种（例如）保留了鞭毛，而属则失去了鞭毛。去年对无柄菌门的前两个基因组（和）进行了测序并发表。BLASTP搜索显示没有TPPP，但具有假纤毛的有一个TPPP。这个TPPP是“长型”，主要出现在动物以及其他后鞭毛生物中。有一个“真菌型”TPPP，仅在一些有鞭毛的真菌中发现。这些关于无柄菌门TPPP蛋白的数据加强了含p25α结构域的蛋白质的出现与真核生物鞭毛/纤毛的出现之间的相关性。
