Rosolanka Robert, Liptak Peter, Baranovicova Eva, Bobcakova Anna, Vysehradsky Robert, Duricek Martin, Kapinova Andrea, Dvorska Dana, Dankova Zuzana, Simekova Katarina, Lehotsky Jan, Halasova Erika, Banovcin Peter
Clinic of Infectology and Travel Medicine, Jessenius Faculty of Medicine in Martin (JFM CU), University Hospital in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia.
Clinic of Internal Medicine-Gastroenterology, Jessenius Faculty of Medicine in Martin (JFM CU), University Hospital in Martin, Comenius University in Bratislava, 036 01 Martin, Slovakia.
Metabolites. 2023 Feb 28;13(3):364. doi: 10.3390/metabo13030364.
Metabolomics is a relatively new research area that focuses mostly on the profiling of selected molecules and metabolites within the organism. A SARS-CoV-2 infection itself can lead to major disturbances in the metabolite profile of the infected individuals. The aim of this study was to analyze metabolomic changes in the urine of patients during the acute phase of COVID-19 and approximately one month after infection in the recovery period. We discuss the observed changes in relation to the alterations resulting from changes in the blood plasma metabolome, as described in our previous study. The metabolome analysis was performed using NMR spectroscopy from the urine of patients and controls. The urine samples were collected at three timepoints, namely upon hospital admission, during hospitalization, and after discharge from the hospital. The acute COVID-19 phase induced massive alterations in the metabolic composition of urine was linked with various changes taking place in the organism. Discriminatory analyses showed the feasibility of successful discrimination of COVID-19 patients from healthy controls based on urinary metabolite levels, with the highest significance assigned to citrate, Hippurate, and pyruvate. Our results show that the metabolomic changes persist one month after the acute phase and that the organism is not fully recovered.
代谢组学是一个相对较新的研究领域，主要侧重于对生物体内选定分子和代谢物进行分析。严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染本身可导致受感染个体的代谢物谱发生重大紊乱。本研究的目的是分析新冠肺炎急性期患者尿液中的代谢组学变化，以及恢复期感染后约一个月的代谢组学变化。我们将讨论观察到的变化与我们之前研究中描述的血浆代谢组变化所导致的改变之间的关系。使用核磁共振波谱对患者和对照组的尿液进行代谢组分析。尿液样本在三个时间点采集，即入院时、住院期间和出院后。新冠肺炎急性期导致尿液代谢成分发生大量改变，这与生物体内发生的各种变化有关。判别分析表明，基于尿液代谢物水平成功区分新冠肺炎患者和健康对照是可行的，其中柠檬酸、马尿酸盐和丙酮酸的显著性最高。我们的结果表明，急性期后一个月代谢组学变化仍然存在，且生物体尚未完全恢复。
