Althof Nadine, Trojnar Eva, Johne Reimar
German Federal Institute for Risk Assessment, Max-Dohrn-Straße 8-10, 10589 Berlin, Germany.
Microorganisms. 2023 Feb 24;11(3):566. doi: 10.3390/microorganisms11030566.
Rotavirus A (RVA) is an important cause of diarrhea in humans and animals. However, RVA in wild animals has only scarcely been investigated so far. Here, the presence of RVA in wild ungulates hunted between 2019 and 2022 in Brandenburg, Germany, was investigated using real-time RT-PCR and sequencing of RT-PCR products. By analyzing intestinal contents, RVA-RNA was detected in 1.0% (2/197) of wild boar (), 1.3% (2/152) of roe deer (), and 2.1% (2/95) of fallow deer () but not in 28 red deer () samples. Genotyping identified G3P[13] strains in wild boar, which were closely related to previously described pig and wild boar strains. Genotype G10P[15] strains, closely related to strains from roe deer, sheep, or cattle, were found in roe deer. The strains of fallow deer represented genotype G3P[3], clustering in a group containing different strains from several hosts. The results indicated a low prevalence of RVA in wild ungulates in Germany. Associations of specific genotypes with certain ungulate species seem to exist but should be confirmed by analyses of more samples in the future.
A组轮状病毒（RVA）是人和动物腹泻的重要病因。然而，迄今为止，野生动物中的RVA鲜有研究。在此，利用实时逆转录聚合酶链反应（RT-PCR）及RT-PCR产物测序，对2019年至2022年在德国勃兰登堡猎获的野生有蹄类动物中RVA的存在情况进行了调查。通过分析肠道内容物，在1.0%（2/197）的野猪、1.3%（2/152）的狍和2.1%（2/95）的黇鹿中检测到RVA-RNA，但在28份马鹿样本中未检测到。基因分型鉴定出野猪中的G3P[13]毒株，它们与先前描述的猪和野猪毒株密切相关。在狍中发现了与来自狍、绵羊或牛的毒株密切相关的G10P[15]基因型毒株。黇鹿的毒株代表G3P[3]基因型，聚集在一个包含来自多个宿主的不同毒株的组中。结果表明德国野生有蹄类动物中RVA的流行率较低。特定基因型与某些有蹄类动物物种之间似乎存在关联，但未来应通过对更多样本的分析加以证实。
