Smith C C, Wilson A P, Prichard B N, Betteridge D J
Clin Sci (Lond). 1986 May;70(5):495-500. doi: 10.1042/cs0700495.
Using high-performance liquid chromatography with electrochemical detection, we have studied the release of endogenous catecholamines from washed platelets induced to aggregate by ADP and thrombin. Washed platelets exhibit irreversible aggregation with 10 mumol/l ADP and 0.3 unit of thrombin/ml, extents of aggregation being 27% and 76% respectively. ADP (10 mumol/l) increased noradrenaline release to the medium by 20% and adrenaline by 28%. As observed with aggregation, 0.3 unit of thrombin/ml produced a more marked effect on release than ADP, noradrenaline and adrenaline being increased by 570% and 169% respectively. Values for platelet noradrenaline content were found to mirror those for the release from platelets induced by thrombin. A correlation was observed between catecholamine release and the concentration of thrombin present in incubations. These data reflect the changes observed with platelet aggregation. This is the first study to determine catecholamine release from platelets by direct measurement. Local catecholamine release after platelet aggregation in vivo may have important consequences for tissue perfusion.
利用高效液相色谱-电化学检测法，我们研究了经ADP和凝血酶诱导聚集的洗涤血小板中内源性儿茶酚胺的释放情况。洗涤血小板与10 μmol/L ADP和0.3单位凝血酶/毫升呈现不可逆聚集，聚集程度分别为27%和76%。10 μmol/L ADP使去甲肾上腺素向培养基中的释放增加了20%，肾上腺素增加了28%。如聚集情况所示，0.3单位凝血酶/毫升对释放的影响比ADP更显著，去甲肾上腺素和肾上腺素分别增加了570%和169%。发现血小板去甲肾上腺素含量的值与凝血酶诱导的血小板释放值一致。观察到儿茶酚胺释放与孵育液中凝血酶浓度之间存在相关性。这些数据反映了血小板聚集时观察到的变化。这是第一项通过直接测量来确定血小板中儿茶酚胺释放的研究。体内血小板聚集后局部儿茶酚胺释放可能对组织灌注有重要影响。
