Wilson A P, Smith C C, Prichard B N, Betteridge D J
Clin Sci (Lond). 1987 Jul;73(1):99-103. doi: 10.1042/cs0730099.
We have used high-performance liquid chromatography with electrochemical detection to measure plasma and platelet catecholamines in 24 normal subjects. In the same subjects platelet function was assessed by measuring platelet aggregation in response to adenosine 5'-pyrophosphate, thrombin, adrenaline and collagen. Platelet sensitivity to prostacyclin was also examined. Platelet noradrenaline showed a positive correlation with extent of aggregation induced by 'low-dose' collagen (1 microgram/ml). No correlation was seen at the higher collagen concentration. Platelet noradrenaline content also correlated with sensitivity of platelets to prostacyclin. High platelet noradrenaline concentrations appeared to result in decreased sensitivity to prostacyclin. No other correlations were observed. These data suggest that platelet noradrenaline rather than plasma levels may be involved in modifying platelet function in vivo. Local release of platelet catecholamines may affect the platelet/vessel wall interaction, the primary physiological step in platelet activation.
我们采用高效液相色谱法结合电化学检测，对24名正常受试者的血浆和血小板儿茶酚胺进行了测定。在同一批受试者中，通过测量血小板对5'-二磷酸腺苷、凝血酶、肾上腺素和胶原蛋白的聚集反应来评估血小板功能。还检测了血小板对前列环素的敏感性。血小板去甲肾上腺素与“低剂量”胶原蛋白（1微克/毫升）诱导的聚集程度呈正相关。在较高胶原蛋白浓度下未观察到相关性。血小板去甲肾上腺素含量也与血小板对前列环素的敏感性相关。高血小板去甲肾上腺素浓度似乎导致对前列环素的敏感性降低。未观察到其他相关性。这些数据表明，血小板去甲肾上腺素而非血浆水平可能参与了体内血小板功能的调节。血小板儿茶酚胺的局部释放可能会影响血小板/血管壁的相互作用，这是血小板激活的主要生理步骤。
