复发性扁桃体炎中存在的苯唑西林/头孢西丁耐药性检测

Detection of Oxacillin/Cefoxitin Resistance in Present in Recurrent Tonsillitis.

作者信息

Messias Aline Cristine Magalhães Costa, Gama Aline Rodrigues, de Almeida Prado Letícia Suriano, da Silva Paulo Alex Neves, Galvão Filho Arlindo Rodrigues, Coelho Clarimar José, Avelino Melissa Ameloti Gomes, Vieira José Daniel Gonçalves, Carneiro Lilian Carla

机构信息

Institute of Tropical Pathology and Public Health, Federal University of Goiás, 235 Street, Goiânia 74605-020, GO, Brazil.

Faculty of Medicine, Federal University of Goiás, 235 Street, Goiânia 74690-900, GO, Brazil.

出版信息

Microorganisms. 2023 Feb 28;11(3):615. doi: 10.3390/microorganisms11030615.

DOI:10.3390/microorganisms11030615

PMID:36985189

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10055619/

Abstract

BACKGROUND

Recurrent tonsillitis is one of the most common diseases in childhood, caused many times by ß-lactam-resistant . The objective of this study was to investigate an alternative method to identify resistance to oxacillin/cefoxitin in from hospitalized children with recurrent tonsillitis.

METHODS

The samples of came from patients with recurrent tonsillitis and were used in 16S rRNA sequencing and an antibiogram test for identification and verifying resistance, after which HSI methodology were applied for separation of resistances.

RESULTS

The isolated showed sensitivity to oxacillin/cefoxitin and the diagnostic images show a visual description of the resistance different groups formed, that may be related to sensitivity and resistance to oxacillin/cefoxitin, characterizing the MRSA .

CONCLUSIONS

Samples that showed phenotypic resistance to oxacillin/cefoxitin were clearly separated from samples that did not show this resistance. A PLS-DA model predicted the presence of resistance to oxacillin/cefoxitin in samples and it was possible to observe the pixels classified as MRSA. The HSI was able to successfully discriminate samples in replicas that were sensitive and resistant, based on the calibration model it received.

摘要

背景

复发性扁桃体炎是儿童期最常见的疾病之一，多次由对β-内酰胺耐药引起。本研究的目的是研究一种替代方法，以鉴定复发性扁桃体炎住院儿童中对苯唑西林/头孢西丁的耐药性。

方法

样本来自复发性扁桃体炎患者，用于16S rRNA测序和抗菌谱测试以进行鉴定和验证耐药性，之后应用HSI方法分离耐药性。

结果

分离出的菌株对苯唑西林/头孢西丁敏感，诊断图像显示了所形成的不同耐药组的直观描述，这可能与对苯唑西林/头孢西丁的敏感性和耐药性有关，从而对耐甲氧西林金黄色葡萄球菌进行了表征。

结论

对苯唑西林/头孢西丁表现出表型耐药性的样本与未表现出这种耐药性的样本明显分开。一个偏最小二乘判别分析（PLS-DA）模型预测了样本中对苯唑西林/头孢西丁耐药性的存在，并且可以观察到分类为耐甲氧西林金黄色葡萄球菌的像素。基于所接收的校准模型，高光谱成像（HSI）能够成功地区分敏感和耐药复制品中的样本。