Zhang Zhen, Chang Lu, Liu Xiuxiu, Wang Jing, Ge Xianhong, Cheng Jiasen, Xie Jiatao, Lin Yang, Fu Yanping, Jiang Daohong, Chen Tao
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Microorganisms. 2023 Mar 11;11(3):724. doi: 10.3390/microorganisms11030724.
Rhizosphere microbiota is important for plant growth and health. Domestication is a process to select suitable plants to satisfy the needs of humans, which may have great impacts on the interaction between the host and its rhizosphere microbiota. Rapeseed ) is an important oilseed crop derived from the hybridization between and ~7500 years ago. However, variations in rhizosphere microbiota along with rapeseed domestication remain poorly understood. Here, we characterized the composition and structure of the rhizosphere microbiota among diverse rapeseed accessions, including ten , two , and three accessions through bacterial 16S rRNA gene sequencing. exhibited a higher Shannon index and different bacterial relative abundance compared with its wild relatives in rhizosphere microbiota. Moreover, artificial synthetic lines G3D001 and No.2127 showed significantly different rhizosphere microbiota diversity and composition from other accessions and their ancestors. The core rhizosphere microbiota of and its wild relatives was also described. FAPROTAX annotation predicted that the synthetic lines had more abundant pathways related to nitrogen metabolism, and the co-occurrence network results demonstrated that acted as hub nodes to promote nitrogen metabolism in the synthetic lines. This study provides new insights into the impacts of rapeseed domestication on the diversity and community structure of rhizosphere microbiota, which may highlight the contribution of rhizosphere microbiota to plant health.
根际微生物群对植物生长和健康至关重要。驯化是一个选择合适植物以满足人类需求的过程，这可能对宿主与其根际微生物群之间的相互作用产生重大影响。油菜是一种重要的油料作物，约在7500年前由甘蓝和白菜杂交而来。然而，关于根际微生物群随油菜驯化的变化仍知之甚少。在此，我们通过细菌16S rRNA基因测序，对不同油菜种质（包括10份甘蓝型油菜、2份白菜型油菜和3份芥菜型油菜种质）的根际微生物群的组成和结构进行了表征。甘蓝型油菜在根际微生物群中表现出更高的香农指数和不同的细菌相对丰度，与其野生近缘种相比。此外，人工合成甘蓝型油菜品系G3D001和2127号的根际微生物群多样性和组成与其他甘蓝型油菜种质及其祖先有显著差异。还描述了甘蓝型油菜及其野生近缘种的核心根际微生物群。FAPROTAX注释预测，合成甘蓝型油菜品系具有更丰富的与氮代谢相关的途径，共现网络结果表明，甘蓝型油菜在合成甘蓝型油菜品系中作为促进氮代谢的枢纽节点。本研究为油菜驯化对根际微生物群多样性和群落结构的影响提供了新的见解，这可能突出了根际微生物群对植物健康的贡献。
