Simos Yannis V, Zerikiotis Stelios, Lekkas Panagiotis, Athinodorou Antrea-Maria, Zachariou Christianna, Tzima Christina, Assariotakis Alexandros, Peschos Dimitrios, Tsamis Konstantinos, Halabalaki Maria, Ververidis Filippos, Trantas Emmanouil A, Economou Garyfalia, Tarantilis Petros, Vontzalidou Argyro, Vallianatou Irini, Angelidis Charalambos, Vezyraki Patra
Laboratory of Physiology, Department of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece.
Laboratory of Agronomy, Department of Crop Science, Agricultural University of Athens, 11855 Athens, Greece.
Microorganisms. 2023 Mar 17;11(3):770. doi: 10.3390/microorganisms11030770.
Several natural compounds have been explored as immune-boosting, antioxidant and anti-inflammatory dietary supplements. Amongst them, hydroxytyrosol, a natural antioxidant found in olive products, and endemic medicinal plants have attracted the scientific community's and industry's interest. We investigated the safety and biological activity of a standardised supplement containing 10 mg of hydroxytyrosol synthesized using genetically modified strains and equal amounts (8.33 μL) of essential oils from subsp. , and in an open-label, single-arm, prospective clinical study. The supplement was given to 12 healthy subjects, aged 26-52, once a day for 8 weeks. Fasting blood was collected at three-time points (weeks 0, 8 and follow-up at 12) for analysis, which included full blood count and biochemical determination of lipid profile, glucose homeostasis and liver function panel. Specific biomarkers, namely homocysteine, oxLDL, catalase and total glutathione (GSH) were also studied. The supplement induced a significant reduction in glucose, homocysteine and oxLDL levels and was tolerated by the subjects who reported no side effects. Cholesterol, triglyceride levels and liver enzymes remained unaffected except for LDH. These data indicate the supplement's safety and its potential health-beneficial effects against pathologic conditions linked to cardiovascular disease.
几种天然化合物已被研究作为增强免疫力、抗氧化和抗炎的膳食补充剂。其中，羟基酪醇，一种存在于橄榄产品中的天然抗氧化剂，以及地方药用植物引起了科学界和产业界的兴趣。我们在一项开放标签、单臂、前瞻性临床研究中，调查了一种标准化补充剂的安全性和生物活性，该补充剂含有10毫克使用转基因菌株合成的羟基酪醇以及等量（8.33微升）来自亚种、和的精油。该补充剂给予12名年龄在26至52岁的健康受试者，每天一次，共8周。在三个时间点（第0周、第8周和第12周随访）采集空腹血液进行分析，分析内容包括全血细胞计数以及血脂、血糖稳态和肝功能指标的生化测定。还研究了特定的生物标志物，即同型半胱氨酸、氧化型低密度脂蛋白、过氧化氢酶和总谷胱甘肽（GSH）。该补充剂使葡萄糖、同型半胱氨酸和氧化型低密度脂蛋白水平显著降低，且受试者能够耐受，未报告有副作用。除乳酸脱氢酶外，胆固醇、甘油三酯水平和肝酶均未受影响。这些数据表明该补充剂的安全性及其对与心血管疾病相关的病理状况的潜在健康益处。
