Environmental Analytical Chemistry Group, Department of Chemistry, University of the Balearic Islands, 07122 Palma, Spain.
Department of Electrical and Electronic Engineering, Universidad del Valle, Cali 760042, Colombia.
Molecules. 2023 Mar 8;28(6):2465. doi: 10.3390/molecules28062465.
The development of a portable device created by 3D printing for colorimetric and fluorometric measurements is an efficient tool for analytical applications in situ or in the laboratory presenting a wide field of applications in the environmental and food field. This device uses a light-emitting diode (LED) as radiation source and a webcam as a detector. Digital images obtained by the interaction between the radiation source and the sample were analyzed using a programming language developed in Matlab (Mathworks Inc., Natick, MA, USA), which builds the calibration curves in real-time using the RGB colour model. In addition, the entire system is connected to a notebook which serves as an LED and detector power supply without the need for any additional power source. The proposed device was used for the determination in situ of norfloxacin, allura red, and quinine in water and beverages samples, respectively. For the validation of the developed system, the results obtained were compared with a conventional spectrophotometer and spectrofluorometer respectively with a -test at a 95% confidence level, which provides satisfactory precision and accuracy values.
3D 打印便携式比色和荧光测量设备的开发是一种用于现场或实验室分析应用的有效工具，在环境和食品领域具有广泛的应用。该设备使用发光二极管 (LED) 作为辐射源，网络摄像头作为检测器。通过辐射源和样品之间的相互作用获得的数字图像使用在 Matlab（Mathworks Inc.，Natick，MA，USA）中开发的编程语言进行分析，该语言使用 RGB 颜色模型实时构建校准曲线。此外，整个系统连接到一个笔记本电脑，该电脑可作为 LED 和检测器的电源，无需任何其他电源。所提出的设备分别用于现场测定水中的诺氟沙星、诱惑红和奎宁以及饮料样品中的这些物质。为了验证所开发系统的有效性，分别使用传统分光光度计和荧光分光光度计与 t 检验（置信水平为 95%）对结果进行了比较，结果提供了令人满意的精密度和准确度值。
