University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland; University of Warsaw, MISMaP College, Banacha 2C, 02-097 Warsaw, Poland.
University of Warsaw, Faculty of Chemistry, Pasteura 1, 02-093 Warsaw, Poland.
Talanta. 2019 Jun 1;198:169-178. doi: 10.1016/j.talanta.2019.01.092. Epub 2019 Jan 28.
Universal, customizable design of 3D printed photometric, and fluorometric flow-through detectors have been presented. The developed designs were fabricated with the use of the most affordable 3D printing technique, namely Fused Filament Fabrication, and require neither hardware nor tools to assemble. Numerous variants of detector geometries have also been presented. The designed parameters varied both in aperture (i.e., the internal diameter of the flow channel in an optical path) and in thickness of an absorbing layer. As expected, the geometry of the channels resulted in changes in the internal volumes. Two concepts of fluorometric detectors have also been described. The utility of all developed flow-through detectors was proven with the use of mechanized calibrations of both photometric and fluorometric experiments. Analytical parameters were characterized with the use of two model dyes: bromothymol blue and fluorescein for photometric and fluorometric experiments, respectively. The repeatability of the 3D printed vessels was found at 3.5-8.0% of the mean relative standard deviation (RSD), depending on the construction of the vessel, which is comparable to rather expensive commercially available flow cells. The compatibility of used 3D printing materials was also examined. For both variants of detection light emitting diodes were applied as light emitters. As the light detectors, both CCD spectrophotometers and light-emitting diodes were used.
本文提出了一种通用的、可定制的 3D 打印光电和荧光流通式检测器设计。所开发的设计使用最实惠的 3D 打印技术，即熔融沉积制造（Fused Filament Fabrication），并且在组装过程中既不需要硬件也不需要工具。还提出了许多不同的检测器几何形状变体。设计的参数不仅在孔径（即在光路中的流动通道的内径）方面有所不同，而且在吸收层的厚度方面也有所不同。正如预期的那样，通道的几何形状导致内部体积发生变化。还描述了两种荧光检测器的概念。使用机械校准的光电和荧光实验，证明了所有开发的流通式检测器的实用性。使用两种模型染料：溴百里酚蓝和荧光素分别进行了光度和荧光实验，对分析参数进行了特征描述。3D 打印容器的重复性在平均相对标准偏差（RSD）的 3.5-8.0%之间，具体取决于容器的结构，这与相当昂贵的商业可用流通池相当。还检查了所用 3D 打印材料的兼容性。对于两种检测变体，都使用发光二极管作为发光体。作为光探测器，同时使用 CCD 分光光度计和发光二极管。
