心材中多酚类化合物的神经保护和抗疱疹特性。
Neuroprotective and Antiherpetic Properties of Polyphenolic Compounds from Heartwood.
机构信息
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 690022 Vladivostok, Russia.
G.P. Somov Institute of Epidemiology and Microbiology, Rospotrebnadzor, 690087 Vladivostok, Russia.
出版信息
Molecules. 2023 Mar 13;28(6):2593. doi: 10.3390/molecules28062593.
In this study, we isolated a new isoflavanostilbene maackiapicevestitol () as a mixture of two stable conformers and as well as five previously known dimeric and monomeric stilbens: piceatannol (), maackin (), scirpusin A (), maackiasine (), and maackolin () from heartwood, using column chromatography on polyamide, silicagel, and C-18. The structures of these compounds were elucidated by NMR, HR-MS, and CD techniques. Maksar obtained from heartwood and polyphenolics - possessed moderate anti-HSV-1 activity in cytopathic effect (CPE) inhibition and RT-PCR assays. A model of PQ-induced neurotoxicity was used to study the neuroprotective potential of polyphenolic compounds from . Maksar showed the highest neuroprotective activity and increased cell viability by 18% at a concentration of 10 μg/mL. Maackolin () also effectively increased the viability of PQ-treated Neuro-2a cells and the value of mitochondrial membrane potential at concentrations up to 10 μΜ. Maksar and compounds - possessed higher FRAP and DPPH-scavenging effects than quercetin. However, only compounds and at concentrations of 10 μM as well as Maksar (10 μg/mL) statistically significantly reduced the level of intracellular ROS in PQ-treated Neuro-2a cells.
在这项研究中，我们从心材中分离出一种新的异黄酮二苯乙烯 maackiapicevestitol ()，它是两种稳定构象 和 的混合物，以及五种先前已知的二聚体和单体二苯乙烯：piceatannol ()、maackin ()、scirpusin A ()、maackiasine ()和 maackolin ()，使用聚酰胺、硅胶和 C-18 柱层析进行分离。这些化合物的结构通过 NMR、HR-MS 和 CD 技术阐明。从心材和多酚中获得的 Maksar 在细胞病变效应 (CPE) 抑制和 RT-PCR 测定中具有中等抗 HSV-1 活性。使用 PQ 诱导的神经毒性模型研究了多酚化合物对 的神经保护潜力。Maksar 在 10 μg/mL 浓度下表现出最高的神经保护活性，使细胞活力提高了 18%。Maackolin () 也能有效提高 PQ 处理的 Neuro-2a 细胞活力和线粒体膜电位值，浓度高达 10 μM。Maksar 和化合物 - 具有比槲皮素更高的 FRAP 和 DPPH 清除作用。然而，只有化合物 和 ，在 10 μM 的浓度下，以及 Maksar（10 μg/mL），在统计学上显著降低了 PQ 处理的 Neuro-2a 细胞内 ROS 水平。
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