N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000 Saint Petersburg, Russia.
Department of Pharmacy and Pharmacology, School of Biomedicine, Far Eastern Federal University, Sukhanova 8, 690950 Vladivostok, Russia.
Molecules. 2023 Feb 21;28(5):2026. doi: 10.3390/molecules28052026.
Three types of extraction were used to obtain biologically active substances from the heartwood of : supercritical CO extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yield of biologically active substances. Several experimental conditions were investigated in the pressure range of 50-400 bar, with 2% of ethanol as co-solvent in the liquid phase at a temperature in the range of 31-70 °C. The most effective extraction conditions are: pressure of 100 bar and a temperature of 55 °C for heartwood. The heartwood of contains various polyphenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI-ion trap) was applied to detect target analytes. High-accuracy mass spectrometric data were recorded on an ion trap equipped with an ESI source in the modes of negative and positive ions. The four-stage ion separation mode was implemented. Sixty-six different biologically active components have been identified in extracts. Twenty-two polyphenols were identified for the first time in the genus .
采用超临界 CO2 萃取、乙醇浸渍和甲醇浸渍三种方法从心材中提取生物活性物质。超临界萃取方法被证明是最有效的萃取方法，能够获得最高产率的生物活性物质。在压力范围为 50-400 巴、液相中含有 2%乙醇作为共溶剂、温度范围为 31-70°C 的条件下，对几种实验条件进行了研究。对于心材，最有效的萃取条件为：压力为 100 巴，温度为 55°C。的心材含有各种多酚化合物和具有有价值生物活性的其他化学基团的化合物。串联质谱（HPLC-ESI-离子阱）用于检测目标分析物。在配备 ESI 源的离子阱中，以负离子和正离子模式记录高精度质谱数据。实施了四阶段离子分离模式。从 提取物中鉴定出 66 种不同的生物活性成分。首次在心材中鉴定出 22 种多酚。
